Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Glucocorticoid action on the immune system.

M M Compton1, L A Caron, J A Cidlowski

  • 1Department of Physiology, University of North Carolina, Chapel Hill 27514.

Journal of Steroid Biochemistry
|January 1, 1987
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

microRNA-17-5p modulates ventral hippocampal transcriptome and synaptic proteome: Implications for emotional regulation in adult male rats.

Neurobiology of disease·2025
Same author

Correction: MiR-16 mediates trastuzumab and lapatinib response in ErbB-2-positive breast and gastric cancer via its novel targets CCNJ and FUBP1.

Oncogene·2023
Same author

MiR-16 mediates trastuzumab and lapatinib response in ErbB-2-positive breast and gastric cancer via its novel targets CCNJ and FUBP1.

Oncogene·2016
Same author

T-cell development of resistance to apoptosis is driven by a metabolic shift in carbon source and altered activation of death pathways.

Cell death and differentiation·2015
Same author

Essential versus accessory aspects of cell death: recommendations of the NCCD 2015.

Cell death and differentiation·2014
Same author

Selective glucocorticoid receptor translational isoforms reveal glucocorticoid-induced apoptotic transcriptomes.

Cell death & disease·2013

Glucocorticoids induce DNA degradation in lymphocytes via receptor-mediated pathways. This process involves the induction of specific nucleases, ultimately leading to programmed cell death (lymphocytolysis).

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • Glucocorticoids significantly impact immune function, partly through steroid-induced cell death.
  • Understanding the mechanism of lymphocytolysis is crucial for immune regulation research.

Purpose of the Study:

  • To elucidate the molecular mechanism of glucocorticoid-induced lymphocytolysis.
  • To identify key factors involved in steroid-mediated lymphocyte death using rat thymocytes.

Main Methods:

  • In vivo administration of glucocorticoids to rats and subsequent analysis of thymocyte DNA.
  • Assessment of cell viability and genomic DNA fragmentation (internucleosomal cleavage).
  • Identification and characterization of nuclear proteins with nuclease activity in treated thymocytes.

Related Experiment Videos

Main Results:

  • Glucocorticoid treatment induced rapid internucleosomal DNA cleavage in lymphocytes, preceding cell death.
  • The antagonist RU 486 blocked glucocorticoid-mediated DNA degradation, indicating receptor involvement.
  • Two families of nucleases were induced by glucocorticoids in thymocytes, directly degrading genomic DNA.

Conclusions:

  • Glucocorticoids trigger lymphocytolysis through a receptor-mediated process.
  • The induction of specific nucleases is a key mechanism in glucocorticoid-induced lymphocyte death.
  • This study proposes a model where steroids activate nucleases, leading to DNA degradation and cell death.