Related Experiment Video
Updated: Oct 7, 2025

Measuring Enzymatic Stability by Isothermal Titration Calorimetry
Published on: March 26, 2019
Parameter Reliability and Understanding Enzyme Function
Andrew G McDonald1, Keith F Tipton1
1School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin, D02 PN40 Dublin, Ireland.
Abstract:
Knowledge of the Michaelis-Menten parameters and their meaning in different circumstances is an essential prerequisite to understanding enzyme function and behaviour. The published literature contains an abundance of values reported for many enzymes. The problem concerns assessing the appropriateness and validity of such material for the purpose to which it is to be applied. This review considers the evaluation of such data with particular emphasis on the assessment of its fitness for purpose.
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