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In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane
Published on: November 28, 2019
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Transcriptomic and Functional Evidence Show Similarities between Human Amniotic Epithelial Stem Cells and
Li-Ping Liu1,2,3, Dong-Xu Zheng3,4, Zheng-Fang Xu5
1Institute of Regenerative Medicine, Affiliated Hospital of Jiangsu University, Jiangsu University, Zhenjiang 212001, China.
Cells
|January 11, 2022
Summary
Amniotic epithelial stem cells (AESCs) show promise for skin regeneration, sharing similarities with keratinocytes (KCs) but possessing unique features. Modulating their properties can enhance their potential for clinical skin repair applications.
Area of Science:
- Regenerative Medicine
- Stem Cell Biology
- Tissue Engineering
Background:
- Amniotic epithelial stem cells (AESCs) are explored as alternatives to keratinocytes (KCs) for skin substitutes.
- Clinical use of AESCs is hindered by unclear similarities and differences compared to KCs.
Purpose of the Study:
- To elucidate commonalities and distinctions between AESCs and KCs using transcriptomics and functional assays.
- To identify strategies for modulating AESCs to improve their suitability for skin regeneration.
Main Methods:
- RNA-sequencing for transcriptomic analysis.
- Functional evaluations in 2D and 3D co-culture systems.
- Investigating the role of TP63 and NOTCH signaling pathways.
Main Results:
- AESCs share epidermis-associated biological processes with KCs, resembling immature KCs.
- AESCs exhibit heterogeneity, with some displaying mesenchymal features distinct from KCs.
- AESCs can phagocytose melanosomes, potentially aiding in pigmented skin reconstitution.
- TP63 overexpression and NOTCH activation enhance AESC stemness and differentiation, respectively.
Conclusions:
- AESCs possess characteristics suitable for skin regeneration, with potential for improvement.
- Modulating AESC properties can bridge the gap between AESCs and KCs, facilitating cell fate convergence.
- Reprogramming strategies may further enhance human AESCs for advanced skin regeneration therapies.
Keywords:
TP63amniotic epithelial stem cellscell fatekeratinocytesmesenchymalreprogrammingskin regenerationskin substitutesstemnesstranscriptomicsMore Related Videos
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