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Updated: Oct 7, 2025

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
Published on: July 3, 2018
Association between Exosomal miRNAs and Coronary Artery Disease by Next-Generation Sequencing
Sheng-Nan Chang1, Jien-Jiun Chen1, Jo-Hsuan Wu2
1Division of Cardiology, Department of Internal Medicine, National Taiwan University Hospital Yunlin Branch, Douliu City 640203, Taiwan.
Background:
Among various bio-informative molecules transferred by exosomes between cells, micro RNAs (miRNAs), which remain remarkably stable even after freeze-and-thaw cycles, are excellent candidates for potential biomarkers for coronary artery disease (CAD).
Methods:
Blood samples were collected from the coronary arteries of 214 patients diagnosed with three-vessel CAD and 140 without CAD. After precipitation extraction, the amounts of exosomes were found to decrease with increased age and three-vessel CAD. Next-generation sequencing was performed to further explore the possible relationship between exosomal miRNAs and CAD.
Results:
Eight exosomal miRNAs showed altered expression associated with CAD. The up-regulated miRNAs in CAD were miRNA-382-3p, miRNA-432-5p, miRNA-200a-3p, and miRNA-3613-3p. The down-regulated miRNAs were miRNA-125a-5p, miRNA-185-5p, miRNA-151a-3p, and miRNA-328-3p.
Conclusion:
We successfully demonstrated particular exosomal miRNAs that may serve as future biomarkers for CAD.
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