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Bacteria Inspired Internal Standard SERS Substrate for Quantitative Detection.

Jiawei Liu1,2, Zilan Hong1, Weimin Yang3

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Summary
This summary is machine-generated.

This study introduces biogenetic gold-silver nanoislands as a surface-enhanced Raman spectroscopy (SERS) substrate. This novel SERS substrate uses bacterial biomolecules for accurate, reproducible quantitative detection of various analytes.

Keywords:
SERSinternal standardmicrobial synthesisnoble metalsquantitative detection

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Area of Science:

  • Environmental Science
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Metal-respiring bacteria are used for metal resource recycling via biosynthesized nanoparticles.
  • Limited utilization of biogenetic nanoparticles combined with bacterial advantages hinders advanced applications.
  • Developing novel substrates for sensitive and reproducible analytical detection is crucial.

Purpose of the Study:

  • To utilize biogenetic gold-silver (Au@Ag) nanoislands as a surface-enhanced Raman spectroscopy (SERS) substrate for quantitative detection.
  • To leverage bacterial biomolecules as an internal standard for enhanced SERS reproducibility and accuracy.
  • To demonstrate the versatility of this biogenetic nanosystem for analyzing diverse chemical compounds.

Main Methods:

  • Biosynthesis of Au@Ag nanoislands on metal-respiring bacteria.
  • Application of the biogenetic Au@Ag nanoislands as a SERS substrate.
  • Utilizing inherent bacterial biomolecules (phospholipid, tyrosine, phenylalanine) as internal standards.
  • Quantitative detection of rhodamine 6G, malachite green, and uric acid using SERS.

Main Results:

  • Au@Ag nanoislands exhibited surface plasmon resonance, enhancing Raman signals.
  • Bacterial biomolecules effectively normalized SERS intensity variations across different hot spots.
  • The biogenetic SERS substrate demonstrated high reproducibility due to gene-controlled biomolecules.
  • Successful quantitative detection of target analytes, showcasing analytical method generality.

Conclusions:

  • Biogenetic Au@Ag nanoislands provide a robust and reproducible SERS substrate for quantitative analysis.
  • The integration of bacterial biomolecules as internal standards overcomes SERS hotspot discrepancies.
  • This approach offers a simple yet effective biogenetic nanosystem for addressing challenges in SERS analysis and beyond.