Simultaneously Detecting Monoamine Oxidase A and B in Disease Cell/Tissue Samples Using Paper-Based Devices.

Area of Science:

• Biochemistry
• Analytical Chemistry
• Biomedical Engineering

Background:

• Monoamine oxidases (MAOs), specifically MAO-A and MAO-B, are crucial enzymes involved in neurotransmitter metabolism.
• Imbalances in MAO-A and MAO-B activity are linked to neurological disorders such as Parkinson's disease (PD) and Alzheimer's disease (AD).
• Accurate detection of MAOs is vital for the diagnosis and treatment of these neurodegenerative conditions.

Purpose of the Study:

• To develop a multiplexed detection method for MAO-A and MAO-B.
• To utilize paper-based devices (PBDs) integrated with chemiluminescence (CL) for sensitive MAO detection.
• To establish PBDs as a viable alternative to traditional methods like Western blotting (WB) for protein analysis.

Main Methods:

• Development of a chemiluminescence-based paper assay for simultaneous detection of MAO-A and MAO-B.
• Quantification of MAO-A and MAO-B in human serum samples using the developed PBDs.
• Validation of the PBDs by analyzing MAOs in human cells and tissue samples, comparing results with Western blotting.

Main Results:

• Achieved low detection limits: 5.01 pg/mL for MAO-A and 8.50 pg/mL for MAO-B in human serum.
• Demonstrated that the paper-based assay yields results with the same trend as Western blotting for cell and tissue samples.
• Showcased the capability of PBDs for simultaneous multiplexed detection of MAO-A and MAO-B, unlike WB.

Conclusions:

• The developed paper-based chemiluminescence assay enables sensitive and simultaneous detection of MAO-A and MAO-B.
• This PBD approach is suitable for preliminary early screening of clinical samples for MAOs.
• The paper-based assay presents a promising alternative to Western blotting for multiplexed protein detection in various biological samples.