Effects of biofouling on the uptake of perfluorinated alkyl acids by organic-diffusive gradients in thin films...

While organic-diffusive gradients in thin films (o-DGT) passive samplers have been used to assess organic contaminants in water, the effects of biofouling on accurate analyte quantification by o-DGT are poorly understood. We evaluated the effects of biofouling on the uptake of six common perfluoroalkyl substances (PFAS) using a previously developed polyacrylamide-WAX (weak anion exchange) o-DGT without a filter membrane. Linear uptake (R² > 0.91) over 21 days was observed in fouled samplers. The measured sampling rates (R_s) and accumulated masses of PFAS in pre-fouled o-DGT were significantly lower (p < 0.05, 20-39% relative error) than in control-fouled samplers. However, compared to clean o-DGT (no biofouling), the R_s of most PFAS in control-fouled samplers (i.e., those with clean diffusive and binding gels initially) were not affected by biofouling. Under flowing (∼5.8 cm s^-1) and static conditions, the measured diffusive boundary layer (DBL) thicknesses for clean o-DGT were 0.016 and 0.082 cm, respectively, whereas the effective in situ biofilm thicknesses for fouled o-DGT were 0.018 and 0.14 cm, respectively. These results suggest that biofilm growth does not have significant effects on target PFAS sampling by o-DGT under typical flowing conditions (≥2 cm s^-1). However, rapid surface growth of biofilm on o-DGT deployed in quiescent waters over long periods of time may exacerbate the adverse effects of biofilms, necessitating the estimation of biofilm thickness in situ. This study provides new insights for evaluating the capability of o-DGT samplers when biofilm growth can be significant.