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Ribozymes02:47

Ribozymes

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The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can...
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Visible light-driven i-motif-based DNAzymes.

Tong Yang1, Shuzhen Peng1, Ruidi Zeng1

  • 1Key Laboratory of the Ministry of Education for Advanced Catalysis Materials, Institute of Physical Chemistry, College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, Zhejiang, China.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|January 11, 2022
PubMed
Summary

Researchers developed a novel DNAzyme using i-motif DNA structures and hypericin. This visible light-driven system generates singlet oxygen for catalysis, showing potential for photocatalytic applications and biodevices.

Keywords:
DNAzymesHypericinSinglet oxygenVisible lighti-motif DNApH

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Nanotechnology

Background:

  • DNAzymes offer catalytic capabilities, with G-quadruplex structures being well-explored.
  • I-motif DNA structures, a less-investigated four-stranded DNA formation, present a novel scaffold for DNAzyme development.

Purpose of the Study:

  • To engineer a visible light-driven DNAzyme utilizing human telomeric i-motifs.
  • To investigate the use of hypericin (Hyp) as a photosensitizer and dissolved oxygen as an oxidant within an i-motif DNAzyme framework.

Main Methods:

  • Formation of i-motif structures in acidic conditions with specific thymine overhangs to bind hypericin.
  • Illumination with visible light to excite hypericin, followed by energy transfer to dissolved oxygen to generate singlet oxygen.
  • Utilizing singlet oxygen for substrate oxidation to demonstrate catalytic activity.

Main Results:

  • The i-motif structure with appropriate thymine overhangs effectively bound hypericin, leading to light-induced emission.
  • Excited hypericin facilitated energy transfer to oxygen, producing singlet oxygen (¹O₂).
  • The generated singlet oxygen initiated substrate oxidation, confirming the DNAzyme's catalytic function.

Conclusions:

  • A novel i-motif-based DNAzyme was successfully developed, driven by visible light and utilizing hypericin and oxygen.
  • The DNAzyme's catalytic efficiency can be enhanced with long-lived mediators.
  • This system demonstrates broad applicability in photocatalysis and biodevice development, leveraging the entire visible light spectrum.