A Simple and Applicable Method for Human Platelet Lysate Preparation Using Citrate Blood

Area of Science:

• Biomedical Sciences
• Regenerative Medicine
• Hematology

Background:

• Human platelet lysate (HPL) is a source of growth factors crucial for tissue regeneration.
• Standard HPL preparation often involves cold centrifugation, which can be resource-intensive.

Purpose of the Study:

• To compare platelet growth factor levels in HPL prepared with final centrifugation at 4°C versus 25°C.
• To establish a simplified, accessible method for HPL preparation.

Main Methods:

• Citrated whole blood from 27 healthy volunteers was used.
• Platelet-rich plasma (PRP) was isolated to prepare HPL.
• HPL was divided for final centrifugation at 4°C and 25°C.
• Platelet growth factors were quantified and compared.

Main Results:

• HPL exhibited higher platelet growth factor concentrations than PRP.
• Final centrifugation at 25°C demonstrated non-inferiority in platelet growth factor levels compared to 4°C.
• The 25°C centrifugation method is effective for HPL preparation.

Conclusions:

• A simple, small-volume HPL preparation method (10-15 mL whole blood) was developed.
• Room temperature (25°C) centrifugation is a viable and practical alternative to cold centrifugation for HPL preparation.
• This method enhances laboratory applicability and accessibility for HPL production.