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Two-Channel Bioprotonic Photodetector.

Jessica Soto-Rodríguez1, Zahra Hemmatian2, Jennifer Black2

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Summary

Researchers developed new bioelectronic devices using blue proteorhodopsin (BPR) and green-light-activated deltarhodopsin (HtdR) to convert biological ion currents into electrical signals. These devices enable wavelength-dependent photocurrent production for potential biological cameras.

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Area of Science:

  • Bioelectronics
  • Molecular Engineering
  • Biophysics

Background:

  • Merging biological systems with electronic components necessitates converting ionic currents to electrical signals.
  • Previous work utilized green-light-activated H. turkmenica deltarhodopsin (HtdR) and palladium (Pd) electrodes for signal generation.
  • Proton transport across membranes is a key mechanism for biological signal transduction.

Purpose of the Study:

  • To expand the spectral range of bioelectronic devices by incorporating blue proteorhodopsin (BPR).
  • To engineer BPR for efficient palladium binding and proton delivery to electronic interfaces.
  • To demonstrate wavelength-dependent photocurrent generation using both HtdR and BPR.

Main Methods:

  • Engineering blue proteorhodopsin (BPR) for palladium (Pd) binding and high-level expression in E. coli.
  • Demonstrating proper orientation of the fused Pd-binding domain for proton transfer to Pd/PdHx contacts.
  • Constructing and characterizing HtdR- and BPR-based devices for photocurrent measurement under varying light wavelengths (450-600 nm).

Main Results:

  • Successfully engineered BPR for Pd binding and proton pumping towards Pd/PdHx electrodes.
  • Demonstrated that BPR is a suitable proton pump for expanding the spectral range of bioelectronic devices.
  • Developed HtdR- and BPR-based devices exhibiting photocurrent maxima separated by 37 nm, showing wavelength-dependent photocurrent production.

Conclusions:

  • Blue proteorhodopsin (BPR) expands the spectral capabilities of bioelectronic signal conversion devices.
  • Engineered BPR and HtdR systems enable wavelength-selective photocurrent generation.
  • These advancements pave the way for developing novel biological cameras and sensors.