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Viruses with RNA Genomes

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RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...

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Related Experiment Video

Updated: Oct 7, 2025

Systemic Delivery of MicroRNA Using Recombinant Adeno-associated Virus Serotype 9 to Treat Neuromuscular Diseases in Rodents

Systemic Delivery of MicroRNA Using Recombinant Adeno-associated Virus Serotype 9 to Treat Neuromuscular Diseases in Rodents

Published on: August 10, 2018

Non-viral vectors for RNA delivery.

Yi Yan¹, Xiao-Yu Liu¹, An Lu¹

¹Beijing Key Laboratory of Molecular Pharmaceutics and New Drug Delivery Systems, State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China.

Journal of Controlled Release : Official Journal of the Controlled Release Society

|January 12, 2022

Summary

RNA therapeutics offer a novel approach to disease treatment. Advanced non-viral delivery systems, including lipid nanoparticles, enhance RNA protection and targeted delivery for improved therapeutic outcomes.

Keywords:

Biological barrier Control release Gene therapy Non-viral vector RNA drugs

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Last Updated: Oct 7, 2025

Systemic Delivery of MicroRNA Using Recombinant Adeno-associated Virus Serotype 9 to Treat Neuromuscular Diseases in Rodents

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Published on: August 10, 2018

Production of Double-stranded DNA Ministrings

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Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Published on: February 1, 2019

Area of Science:

Biotechnology
Molecular Biology
Nanomedicine

Background:

RNA-based therapies utilize exogenous nucleic acids like mRNA, siRNA, miRNA, or ASO to modulate gene expression.
The success of mRNA vaccines against COVID-19 highlights the potential of RNA therapeutics.

Purpose of the Study:

To review biological barriers in in vivo RNA delivery.
To discuss advancements in non-viral RNA delivery systems for therapeutic applications.

Main Methods:

Review of current literature on RNA delivery systems.
Analysis of non-viral nanocarriers for RNA protection and targeting.

Main Results:

Non-viral delivery systems effectively protect RNA from degradation by ribonucleases.
Nanoparticles facilitate targeted tissue accumulation, cellular uptake, and controlled release of RNA therapeutics.

Conclusions:

Non-viral delivery systems are crucial for overcoming in vivo RNA delivery challenges.
These advanced systems hold significant promise for the future of RNA-based disease treatment.