Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

From microtissues to macro solutions - The future of scalable and automated cartilage tissue engineering.

Journal of orthopaedic translation·2026

Same author

Mechanical Tuning of the Cell Microenvironment Using a Biomimetic Hydrogel System for Articular Cartilage Tissue Engineering.

Journal of tissue engineering and regenerative medicine·2026

Same author

Efficacy of the spring distraction system for different etiologies of early onset scoliosis: evaluating an evolving treatment concept.

Spine deformity·2026

Same author

A Decade of Leadership and Impact: Celebrating 10 Years of the ORS Spine Section.

JOR spine·2026

Same author

Development and assessment of a novel silk fibroin methacrylate and keratin hydrogel for human hip acetabular labrum regeneration.

Biomaterials advances·2026

Same author

Chondroitinase Versus Papain Digestion Leads to Different Outcome for In Vitro Simulation of Degenerated Discs.

JOR spine·2026

Search research articles

Related Experiment Video

Updated: Oct 7, 2025

Author Spotlight: Enhancing Bone Regeneration with Vascularized Artificial Cartilage Integration

Author Spotlight: Enhancing Bone Regeneration with Vascularized Artificial Cartilage Integration

Published on: July 14, 2023

Evaluating Initial Integration of Cell-Based Chondrogenic Constructs in Human Osteochondral Explants.

Meike W A Kleuskens¹, João F Crispim¹, Corrinus C van Donkelaar¹

¹Orthopaedic Biomechanics, Department of Biomedical Engineering, Eindhoven University of Technology, The Netherlands.

Tissue Engineering. Part C, Methods

|January 12, 2022

Summary

This study demonstrates an ex vivo human osteochondral model for evaluating cartilage implant integration. Organoid-based implants showed successful tissue remodeling and integration, reducing the need for animal testing in cartilage regeneration research.

Keywords:

articular cartilage ex vivo explant integration organoids osteochondral

More Related Videos

Isolation of Chondrocytes and Chondroprogenitors Using Fibronectin Adhesion and Migratory Assay

Isolation of Chondrocytes and Chondroprogenitors Using Fibronectin Adhesion and Migratory Assay

Published on: October 4, 2024

In Vitro Impact Model to Generate Sublethal Chondrocyte Injury in Bovine Cartilage Explants

In Vitro Impact Model to Generate Sublethal Chondrocyte Injury in Bovine Cartilage Explants

Published on: June 27, 2025

Related Experiment Videos

Last Updated: Oct 7, 2025

Author Spotlight: Enhancing Bone Regeneration with Vascularized Artificial Cartilage Integration

Author Spotlight: Enhancing Bone Regeneration with Vascularized Artificial Cartilage Integration

Published on: July 14, 2023

Isolation of Chondrocytes and Chondroprogenitors Using Fibronectin Adhesion and Migratory Assay

Isolation of Chondrocytes and Chondroprogenitors Using Fibronectin Adhesion and Migratory Assay

Published on: October 4, 2024

In Vitro Impact Model to Generate Sublethal Chondrocyte Injury in Bovine Cartilage Explants

In Vitro Impact Model to Generate Sublethal Chondrocyte Injury in Bovine Cartilage Explants

Published on: June 27, 2025

Area of Science:

Biomaterials Science
Tissue Engineering
Regenerative Medicine

Background:

Implant integration is crucial for cartilage regeneration but typically assessed via animal studies.
Reducing animal use and increasing screening efficiency are key goals in implant development.

Purpose of the Study:

To investigate an ex vivo human osteochondral culture model for assessing allogeneic cell-based implant integration.
To evaluate the potential of this model to replace or supplement in vivo studies.

Main Methods:

Chondrocytes were expanded into organoids or standard cell cultures.
Three implant types (fibrin+organoids, fibrin+cells, fibrin only) were used to fill defects in human osteochondral explants.
Explants were cultured for 1 or 28 days in a double-chamber system for analysis.

Main Results:

After 28 days, organoid implants remodeled, merged, and cells migrated, integrating with native cartilage.
Newly formed tissue rich in proteoglycans and collagen type II was observed.
Biochemical analysis showed significant effects of culture time on glycosaminoglycan content and higher hydroxyproline in organoid implants.

Conclusions:

The ex vivo human osteochondral model effectively demonstrates implant integration and matrix production.
This system offers a controlled environment for evaluating cartilage implant strategies, reducing reliance on animal models.
The model aids in exploring and identifying promising cell-based implant approaches for cartilage regeneration.