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Updated: Oct 7, 2025

Segmentation and Measurement of Fat Volumes in Murine Obesity Models Using X-ray Computed Tomography
Published on: April 4, 2012
HELICAL COMPUTERIZED TOMOGRAPHY CAN MEASURE SUBCUTANEOUS, VISCERAL AND TOTAL FAT AREAS?
Guilherme Wendler1,2, Paulo Afonso Nunes Nassif1, Osvaldo Malafaia1
1Postgraduate Program in Principles of Surgery, Mackenzie Evangelical Faculty of Paraná/Medical Research Institute, Curitiba, PR, Brazil.
Abdominal obesity increases total and subcutaneous fat with BMI progression. Visceral fat measurements did not significantly differ across obesity grades in this computed tomography study.
Area of Science:
- Medical Imaging
- Radiology
- Obesity Research
Background:
- Abdominal obesity, characterized by increased abdominal adipose tissue, is a significant risk factor for various health conditions.
- Computed tomography (CT) is a proposed method for quantifying abdominal fat area.
Purpose of the Study:
- To correlate obesity classifications with total, subcutaneous, and visceral fat areas.
- To analyze the relationship between body mass index (BMI) and abdominal fat distribution.
Main Methods:
- Cross-sectional observational study utilizing abdominal CT scans.
- Analysis of a single CT slice including the L4 vertebral body and umbilical scar.
- Measurement of total, visceral, and subcutaneous fat areas using Horos v3.3.5 software.
Main Results:
- Total fat area increased with higher obesity grades (II, III, and superobese).
- Subcutaneous fat area also increased with BMI progression.
- Visceral fat area did not show significant differences between obesity grades, with a trend towards lower averages in grade II obesity.
Conclusions:
- Total and subcutaneous fat areas increase with advancing BMI categories.
- Visceral fat area measurements did not demonstrate a similar increase across BMI groups.
- CT-based quantification of abdominal fat provides insights into obesity-related changes.
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