DNA Framework-Programmed Micronano Hierarchy Sensor Interface for Metabolite Analysis in Whole Blood

Area of Science:

• Biotechnology
• Nanotechnology
• Analytical Chemistry

Background:

• Microsubstrates offer improved sensing but lack nanoscale probe control.
• Precise nanoscale regulation of probes on microscale interfaces is crucial for advanced biosensing.

Purpose of the Study:

• To develop a programmed microscale biosensing interface for metabolite analysis using tetrahedron DNA framework (TDF).
• To enhance the sensitivity and stability of biosensors for detecting metabolites like ATP.

Main Methods:

• Employed tetrahedron DNA framework (TDF) to anchor aptamers on a microscale biosensing interface.
• Utilized square wave voltammetry for interrogating the TDF-aptamer probe's response to ATP.
• Evaluated the performance of the TDF-corbelled aptamer against single-stranded aptamer (SSA).

Main Results:

• The TDF-corbelled aptamer demonstrated approximately 2.8-fold higher signal alteration to ATP compared to SSA.
• The micronano composite probes exhibited high stability and fast response kinetics in complex matrices.
• Achieved a limit of detection for ATP as low as 50 μM in rabbit whole blood.

Conclusions:

• The TDF-programmed microscale biosensing interface significantly enhances metabolite detection sensitivity.
• The developed micronano hierarchy complex shows potential for implantable devices for real-time metabolic monitoring.