Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Interfacial Redox-Driven Crystallization on MXene Enables Ultrasensitive Hg<sup>2+</sup> Detection.

ACS applied materials & interfaces·2026
Same author

Development of CAR NK Cell Lines Selectively Targeting Cancer Cells Expressing Membrane Hsp70.

MedComm·2026
Same author

Azothiophene-based molecular switches: influence of substituent position and solvent environment on photophysical behavior.

Physical chemistry chemical physics : PCCP·2025
Same author

Heat shock protein 70 (Hsp70) as a target for advancing immunotherapy in solid tumors.

Cytokine & growth factor reviews·2025
Same author

Red blood cell Raman microscopy: modelling sub-cellular biochemistry.

Physical chemistry chemical physics : PCCP·2025
Same author

Raman microscopy as a tool to study changes in chemical composition upon structural differentiation of <i>Ambystoma</i> embryo.

Journal of materials chemistry. B·2025

Related Experiment Video

Updated: Oct 7, 2025

How to Study Basement Membrane Stiffness as a Biophysical Trigger in Prostate Cancer and Other Age-related Pathologies or Metabolic Diseases
13:18

How to Study Basement Membrane Stiffness as a Biophysical Trigger in Prostate Cancer and Other Age-related Pathologies or Metabolic Diseases

Published on: September 20, 2016

9.8K

Materials-Based Approach for Interrogating Human Prostate Cancer Cell Adhesion and Migratory Potential Using a

Matthew Nicklin, Graham J Hickman, A Graham Pockley

    ACS Applied Bio Materials
    |January 12, 2022
    PubMed
    Summary

    Prostate cancer cells

    Keywords:
    adhesionepithelialmesenchymalsilicasurface chemistry

    More Related Videos

    Isolation, Culture, and Characterization of Prostate Cancer-Associated Fibroblasts
    09:43

    Isolation, Culture, and Characterization of Prostate Cancer-Associated Fibroblasts

    Published on: August 1, 2025

    269
    3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer
    09:24

    3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer

    Published on: September 13, 2018

    9.1K

    Related Experiment Videos

    Last Updated: Oct 7, 2025

    How to Study Basement Membrane Stiffness as a Biophysical Trigger in Prostate Cancer and Other Age-related Pathologies or Metabolic Diseases
    13:18

    How to Study Basement Membrane Stiffness as a Biophysical Trigger in Prostate Cancer and Other Age-related Pathologies or Metabolic Diseases

    Published on: September 20, 2016

    9.8K
    Isolation, Culture, and Characterization of Prostate Cancer-Associated Fibroblasts
    09:43

    Isolation, Culture, and Characterization of Prostate Cancer-Associated Fibroblasts

    Published on: August 1, 2025

    269
    3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer
    09:24

    3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer

    Published on: September 13, 2018

    9.1K

    Area of Science:

    • Cancer Biology
    • Cell Biology
    • Biomaterials Science

    Background:

    • Prostate cancer heterogeneity involves epithelial and mesenchymal phenotypes.
    • Cancer stem cells (CSCs) contribute to tumor progression.
    • Standard tissue culture plastic (TCP) may not fully represent in vivo cell behavior.

    Purpose of the Study:

    • Investigate the impact of surface chemistry on prostate cancer cell adhesion.
    • Characterize epithelial and mesenchymal prostate cancer cell subpopulations.
    • Explore the role of cell differentiation in adhesion and metastasis.

    Main Methods:

    • Utilized OPCT-1 prostate cancer cell line and derived subpopulations (P4B6B, P5B3).
    • Compared cell adhesion on standard tissue culture plastic (TCP) and fluoroalkylsilica (FS) surfaces.
    • Analyzed cell phenotype and spheroid formation under different culture conditions.

    Main Results:

    • Mesenchymal cells (P4B6B) poorly adhered to FS surfaces, forming spheroids.
    • Epithelial cells (P5B3) formed adherent monolayers on FS surfaces.
    • Mesenchymal cells adhered to FS when co-cultured with epithelial cells, suggesting cell-cell interaction influences adhesion.

    Conclusions:

    • Epithelial-mesenchymal transition (EMT) status significantly impacts cell adhesive capacity.
    • Low surface energy (FS) culture surfaces reveal adhesion differences not seen on TCP.
    • Findings offer insights into cell adhesion mechanisms relevant to prostate cancer metastasis.