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Updated: Oct 7, 2025
Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors
Published on: April 9, 2014
Turn-On Assay for HIV-1 Protease Inhibitor Selection
Yan Shi1, Zhuang Li1, Hongda Wang1
1State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, China.
Abstract:
Virus threatens our lives and health at all times. Among them, HIV has caused millions of deaths since its discovery. Research on viral inhibitor selection has become an urgent need. A reliable turn-on assay for HIV-1 protease (HIV-1 PR) inhibitor selection has been developed. The feasibility assay of protease detection was demonstrated based on fluorescence resonance energy transfer between graphene oxide and dye-modified peptides. Besides the rapid and sensitive detection of HIV-1 PR, the enzyme kinetic parameters were also measured. Most importantly, this assay was applied in the distinction of protease inhibitor efficiency and the unknown inhibitor selection.
