Pulsed Electrodeposition of HAP/CPG-BSA/CUR Nanocomposite on Titanium Metal for Potential Bone Regeneration

Bioactive hydroxyapatite (HAP) composites are progressively predicted as successive materials in bone regeneration therapy. A nanostructured composite made from the mixture of hydroxyapatite, chitosan (C)-polyethylene glycol (P)-gelatin (G), and bovine serum albumin (BSA) (HAP/CPG- BSA) was prepared using ultra probe sonication followed by lyophilization. The anti-inflammatory and antioxidant-rich curcumin (CUR) molecules were impregnated on HAP/CPG-BSA composite for self-repairing bone regeneration. The physicochemical morphology of HAP/CPG-BSA/CUR composite was analyzed by FTIR, XRD, SEM, and TEM techniques. The 92.0% of CUR release was observed from HAP/CPG-BSA/CUR composite by UV-VIS spectroscopy at a λ_max value of 420 nm. The in vitro studies of HAP/CPG-BSA/CUR against osteoblast-like (MG63) cells and fibroblast cells (L929) resulted from 90.0 and 91.0% of cell viability for significant improvement in osteoinduction, proliferation, and viability for bone regeneration. The in vivo results of bone-cell formation by the implantation suggest that HAP/CPG-BSA/CUR composite might be useful in applications of regeneration medicine. The outcome of this study shows the ability to favor bone regeneration and signifies that this may be a prospective substitute implant for elevated repairing of bone.