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DNA Barcode Quantification As a Robust Tool for Measuring Mixing Ratios in Two-Component Systems.

A Xavier Kohll¹, Julian Koch¹, Weida D Chen¹

¹Institute for Chemical and Bioengineering, ETH Zurich, Vladimir-Prelog-Weg 1, Zürich 8093, Switzerland.

ACS Applied Bio Materials

|January 13, 2022

Summary

DNA barcodes, quantified using quantitative real-time polymerase chain reaction (PCR), accurately determine mixing ratios in manufacturing. This method simplifies analysis for liquid and polymeric products without extensive infrastructure.

Keywords:

DNA barcodes mixing multiplex PCR taggant ΔCQ

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Area of Science:

Materials Science
Biotechnology
Analytical Chemistry

Background:

Accurate mixing compositions are vital for product quality in manufacturing.
Traditional component balance analysis for mixing ratios is complex and infrastructure-intensive.
DNA barcodes offer a potential low-cost solution for product authenticity and composition analysis.

Purpose of the Study:

To demonstrate that quantitative real-time polymerase chain reaction (PCR) of DNA barcodes can determine mixing ratios in various products.
To establish a method for distributing DNA barcodes within diverse material matrices.
To validate the accuracy and applicability of DNA barcode quantification for mixture analysis.

Main Methods:

Encapsulating DNA barcodes in silica nanoparticles for matrix distribution.
Utilizing quantitative real-time polymerase chain reaction (PCR) for DNA barcode quantification.
Employing multiplex PCR reactions to determine relative DNA barcode concentrations in mixtures.

Main Results:

Successfully quantified DNA barcodes via quantitative real-time PCR (qPCR) to determine mixing ratios.
Demonstrated applicability across a range of liquid and polymeric products.
Achieved accurate composition determination from 10 parts per million (ppm) down to 10 parts per billion (ppb) initial barcode concentrations.
Applied the method to determine mixing ratios in polysilicon products.

Conclusions:

Quantitative DNA barcode analysis via qPCR is an effective and versatile method for determining mixing ratios.
This technique simplifies the analysis of complex mixtures, reducing the need for extensive infrastructure.
The method is robust across various concentrations and product types, offering a valuable tool for quality control.