Pluronic-Based Nanocarrier Platform Encapsulating Two Enzymes for Cascade Reactions

Area of Science:

• Biotechnology
• Materials Science

Background:

• Enzyme immobilization is crucial for applications like biosensing and synthesis.
• Coimmobilizing multiple enzymes presents challenges in achieving high yield, activity retention, and low leaching.

Purpose of the Study:

• To develop a pluronic-based nanocarrier (PNC) for efficient enzyme immobilization and coimmobilization.
• To evaluate the PNC's performance in terms of encapsulation yield, enzyme activity, and leaching.

Main Methods:

• Utilized a temperature-sensitive pluronic-based nanocarrier (PNC) for enzyme encapsulation.
• Tested six different enzymes for single immobilization and two enzymes (formate dehydrogenase and mannitol dehydrogenase) for coimmobilization.
• Assessed immobilization yield, retained enzymatic activity, and enzyme leaching over time.

Main Results:

• PNC achieved high encapsulation yields (∼90%) and retained high enzymatic activity (>95%) for all tested single enzymes.
• Enzyme leaching was minimal (<0.13% over 2 weeks).
• Coimmobilization of formate dehydrogenase and mannitol dehydrogenase in PNC significantly enhanced d-mannitol production (>10-fold increase) compared to free enzymes.

Conclusions:

• Pluronic-based nanocarriers are effective platforms for single and multiple enzyme immobilization.
• The PNC system offers high efficiency, activity retention, and stability, making it suitable for cascade reactions and other enzyme applications.