Related Experiment Video
Updated: Oct 6, 2025
07:43
Coincubation Assay for Quantifying Competitive Interactions between Vibrio fischeri Isolates
Published on: July 22, 2019
8.3K
The global regulators ArcA and CytR collaboratively modulate Vibrio cholerae motility
Yuehua Li1,2,3, Junxiang Yan1,2,3, Xueqian Guo1,2,3
1TEDA Institute of Biological Sciences and Biotechnology, Nankai University, No.23, Hongda Street, Tianjin Economic and Technological Development Area, Tianjin, 300457, China.
BMC Microbiology
|January 13, 2022
Summary
ArcA regulates Vibrio cholerae motility by repressing flrA expression. It also indirectly controls motility via CytR, which influences flagellar gene fliK expression, impacting bacterial movement.
Area of Science:
- Microbiology
- Bacterial Pathogenesis
- Molecular Biology
Background:
- Vibrio cholerae motility is crucial for pathogenesis, driven by a polar flagellum.
- ArcA, a global anaerobiosis regulator, impacts virulence and biofilm formation but its role in motility is unknown.
- CytR is involved in V. cholerae pathogenesis and flagellar synthesis, yet its precise mechanism in motility remains unclear.
Purpose of the Study:
- To elucidate the role of ArcA in V. cholerae motility.
- To investigate the regulatory mechanism of CytR in V. cholerae motility.
- To understand the interplay between ArcA and CytR in controlling bacterial flagellar expression and motility.
Main Methods:
- Comparative analysis of motility in wild-type (WT) and mutant V. cholerae strains (ΔarcA, ΔcytR).
- Investigation of direct regulatory interactions using gene expression analysis.
- Assessment of the impact of ArcA and CytR on key flagellar gene expression (flrA, fliK).
Main Results:
- The ΔarcA mutant showed increased motility compared to WT, indicating ArcA represses motility.
- ArcA directly represses the expression of flrA, a key motility regulator.
- CytR directly enhances fliK expression, crucial for motility, and its expression is repressed by ArcA.
Conclusions:
- ArcA is essential for regulating V. cholerae motility.
- ArcA directly controls motility by repressing flrA expression.
- ArcA indirectly influences motility by regulating CytR, which in turn affects fliK expression and bacterial movement.
More Related Videos
07:59Investigating Flagella-Driven Motility in Escherichia coli by Applying Three Established Techniques in a Series
Published on: May 10, 2020
7.9K
07:58Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates
Published on: May 30, 2017
11.2K
Related Concept Videos
01:18Flagella and Motility in Bacteria
864
Flagella are specialized, thread-like structures that extend from a bacteria's cell envelope. They play a crucial role in motility and chemotaxis. Their structural organization and functioning exemplify sophisticated biological engineering, enabling bacterial survival and adaptability in diverse environments.Structure of the FlagellumA bacterial flagellum consists of three key components: the filament, the hook, and basal body. The filament, a long, helical structure composed of repeating...
864
01:27Chemotaxis in E. coli
165
Chemotaxis in Escherichia coli is a sensory-driven motility mechanism that enables bacteria to navigate chemical gradients, moving toward beneficial environments while avoiding harmful conditions. This process relies on a signal transduction system integrating external chemical cues with flagellar motor control.Chemoreceptors and Signal DetectionE. coli detects chemical gradients through methyl-accepting chemotaxis proteins (MCPs), which are membrane-bound chemoreceptors that sense attractants...
165
01:10Intracellular Movement of Viruses and Bacteria
3.0K
Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a...
3.0K
01:13Actin Polymerization and Cell Motility
5.6K
Actin is a family of globular proteins that are highly abundant in eukaryotic cells. It makes up approximately 1-5% of total cell protein concentration. Actin monomers polymerize to form a complex network of polarized filaments, the actin cytoskeleton, that plays a crucial role in many cellular processes, including cell motility, division, endocytosis, and metastasis of cancer cells.
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
5.6K
01:16Cell Motility through Blebbing
2.1K
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
Blebbing Through the Matrix
In multicellular...
2.1K
01:09GPCRs Regulate Adenylyl Cylase Activity
6.1K
Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
6.1K