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Updated: Oct 6, 2025

Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection
Published on: November 7, 2018
APOBEC3C S188I Polymorphism Enhances Context-Specific Editing of Hepatitis B Virus Genome
Pierre Khalfi1,2, Rodolphe Suspène1, Vincent Caval1
1Department of Virology, Institut Pasteur, Université de Paris, CNRS UMR3569, Paris, France.
Abstract:
Single-nucleotide polymorphism in APOBEC3C (resulting in a serine to isoleucine in position 188) is present in approximately 10% of African populations and greatly enhances restriction against human immunodeficiency virus-1 and simian immunodeficiency virus by improving dimerization and DNA processivity of the enzyme. In this study, we demonstrated in culture and in infected patients that hepatitis B virus (HBV) could be edited by APOBEC3CS188I. Using next-generation sequencing, we demonstrated that APOBEC3CS188I led to enhanced editing activity in 5'TpCpA→5'TpTpA context. This constitutes a new hallmark of this enzyme, which could be used to determine its impact on HBV or nuclear DNA.
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