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Polyphenols extracted from Shanxi-aged vinegar exert hypolipidemic effects on OA-induced HepG2 cells via the
Jia Song1, Huirui Qiu1, Peng Du1
1Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, State Key Laboratory of Food Nutrition and Safety, College of Biotechnology, Tianjin University of Science & Technology, Tianjin, China.
Abstract:
Hyperlipidemia is one of the key risk factors causing many chronic diseases, and lowering blood lipid levels can prevent many diseases. In this paper, a hyperlipidemic cell model of oleic acid (OA) induced hepatocellular carcinoma cells (HepG2) was established using polyphenols extracted from Shanxi-aged vinegar (SAVEP). The effects of SAVEP on nuclear damage, mitochondrial membrane potential, apoptosis, cellular lipid deposition, and lipid metabolism protein expression in HepG2 hyperlipidemic cells were examined to investigate the lipid-lowering mechanism of SAVEP at the cellular level. The results showed that SAVEP could reduce the content of TC/TG index, repair the nuclear damage, reduce lipid accumulation and finally decrease the rate of apoptosis by up-regulating the expression of key proteins such as PPARα, LXRα, and ABCA1 in the process of lipid metabolism. PRACTICAL APPLICATIONS: In this thesis, the hypolipidemic activity of polyphenol extracts from Shanxi-aged vinegar was analyzed on the level of HepG2 cells. The hypolipidemic mechanism of oxidative stress, lipid metabolism and inflammatory stress was also elucidated. It provided a theoretical basis for the in-depth understanding of the hypolipidemic health effects of Shanxi-aged vinegar.
Insights
Polyphenols from Shanxi-aged vinegar (SAVEP) reduce lipid levels in liver cancer cells. SAVEP improves lipid metabolism and reduces cell damage, offering a potential natural approach for managing hyperlipidemia.
Area of Science:
- Biochemistry
- Cell Biology
- Pharmacology
Background:
- Hyperlipidemia is a major risk factor for chronic diseases.
- Lowering blood lipid levels is crucial for disease prevention.
- Hepatocellular carcinoma (HepG2) cells are used to model hyperlipidemia.
Purpose of the Study:
- To investigate the lipid-lowering effects of Shanxi-aged vinegar polyphenols (SAVEP).
- To elucidate the cellular mechanisms underlying SAVEP's hypolipidemic activity.
- To assess SAVEP's impact on cellular damage and lipid metabolism in HepG2 cells.
Main Methods:
- Established an oleic acid-induced hyperlipidemic HepG2 cell model.
- Treated cells with SAVEP.
- Assessed nuclear damage, mitochondrial membrane potential, and apoptosis.
- Measured cellular lipid deposition and expression of lipid metabolism proteins (PPARα, LXRα, ABCA1).
Main Results:
- SAVEP reduced the total cholesterol/triglyceride (TC/TG) index.
- SAVEP repaired nuclear damage and decreased lipid accumulation.
- SAVEP upregulated key lipid metabolism proteins (PPARα, LXRα, ABCA1), reducing apoptosis.
Conclusions:
- SAVEP exhibits significant hypolipidemic activity in HepG2 cells.
- The mechanism involves regulating lipid metabolism proteins and reducing cellular damage.
- SAVEP provides a theoretical basis for understanding the health benefits of aged vinegar.
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