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A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants
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Published on: February 19, 2016

Au Film-Au@Ag Core-Shell Nanoparticle Structured Surface-Enhanced Raman Spectroscopy Aptasensor for Accurate

Xiaohui Jing1, Lin Chang2, Lixia Shi1

  • 1Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi, Jiangsu 214122, P.R. China.

ACS Applied Bio Materials
|January 13, 2022
PubMed
Summary

A new internal standard (IS) aptasensor offers sensitive detection of the toxin ochratoxin A (OTA). This method uses nanoparticle release and signal ratios for accurate, ratiometric quantification in environmental and food safety applications.

Keywords:
IS aptasensorOTASERScore−shellratiometric

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Area of Science:

  • Environmental Science
  • Food Science
  • Analytical Chemistry
  • Nanotechnology

Background:

  • Ochratoxin A (OTA) is a potent toxin requiring sensitive detection methods.
  • Existing detection methods for OTA may lack sensitivity and quantativity.
  • Development of advanced sensors is crucial for environmental and food safety.

Purpose of the Study:

  • To develop a sensitive and quantitative internal standard (IS) aptasensor for ochratoxin A (OTA) detection.
  • To enhance the reliability and scalability of OTA detection.
  • To provide a robust platform for quantitative SERS measurements.

Main Methods:

  • Synthesized an IS aptasensor using modified aptamers on Au@Ag core-shell nanoparticles and Au films.
  • Incorporated 4-ATP and 4-NTP as internal standards for ratiometric signal analysis.
  • Utilized SERS to monitor nanoparticle release and quantify OTA concentration via the I1078/I1335 ratio.

Main Results:

  • Achieved high consistency in OTA detection with R2 of 0.993 and RSD of 0.94% through IS correction.
  • Established a low OTA detection limit of 5 pM.
  • Demonstrated a ratiometric detection method where the I1078/I1335 ratio accurately reflects OTA concentration.

Conclusions:

  • The developed IS aptasensor provides a reliable and scalable platform for OTA detection.
  • This method offers a continuous and high-throughput approach for quantitative SERS measurements.
  • The aptasensor shows significant promise for detecting various molecules in complex matrices.