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Directed Assembly of Elastin-like Proteins into defined Supramolecular Structures and Cargo Encapsulation In Vitro
Published on: April 8, 2020
Amphiphilic Protein Microfibrils from Ice-Templated Self-Assembly and Disassembly of Pickering Emulsions
1Biological and Agricultural Engineering, University of California, Davis, California 95616, United States.
Abstract:
Amphiphilic protein microfibrils have been generated for the first time by ice-templated self-assembly of aqueous globular protein colloids and subsequent selective disassembly in polar solvents like MeOH, EtOH, acetone, and dimethylformamide. Semicrystalline microfibrils, ca. 1.2 μm wide and 45-70 μm long, produced from soy proteins are excellent amphiphiles, which are capable of stabilizing both high-internal-phase o/w and w1/o/w2 double emulsions as well as retaining amphiphilicity even with surface-bound lipophiles and electrophiles. This ice-templated self-assembling and polar solvent disassembling approach is applicable to other legume proteins, such as pea proteins, and is scalable to process globular proteins into amphiphilic microfibrils for Pickering emulsions in many potential applications including food, pharmaceuticals and skin care.
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