Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

B4GALT5 deficiency impairs glycosphingolipid biosynthesis: a new Congenital Disorder of Glycosylation?

Journal of lipid research·2026
Same author

Integrated Proteomic and Lipidomic Profiling of Aqueous Humor Reveals Inflammatory Signatures in Retinitis Pigmentosa.

Biomedicines·2026
Same author

Comprehensive Profiling of Phytocannabinoids and Semisynthetic Cannabinoids in Seized Materials from Northern Italy.

ACS omega·2026
Same author

Sphingolipidomic Profiling of Peripheral Blood Mononuclear Cells Reveals a Distinct Immunometabolic Signature Across Patients with Essential Obesity and Metabolic Syndrome Compared to Normal-Weight Healthy Subjects.

Journal of clinical medicine·2026
Same author

A potent nonapeptide inhibitor for the CXCL12/HMGB1 heterocomplex: A computational and experimental approach.

Computational and structural biotechnology journal·2026
Same author

In Vivo and In Vitro Evaluation of PTeCA (1H-Pyrrole-2,3,4,5-Tetracarboxylic Acid) in Hair Matrix as a Marker for Oxidative Cosmetic Treatment.

Drug testing and analysis·2026

Related Experiment Video

Updated: Oct 6, 2025

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
11:17

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

Published on: June 1, 2017

35.7K

Tip-tip filtration ameliorates single-phase extraction methods for plasma large-scale lipidomics analysis.

Camillo Morano1, Gabriella Roda2, Rita Paroni1

  • 1Department of Health Sciences, Università degli Studi di Milano, Milan, Italy.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
|January 13, 2022
PubMed
Summary

We optimized lipid extraction for untargeted lipidomics using a methanol/chloroform mixture and a novel tip-tip filtration method. This approach enhances reproducibility and lipid coverage while saving time and money.

Keywords:
Lipid extractionMass spectrometrySample pre-treatmentUntargeted lipidomics

More Related Videos

Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis
05:47

Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis

Published on: June 25, 2020

5.3K
Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery
09:28

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery

Published on: October 27, 2023

3.2K

Related Experiment Videos

Last Updated: Oct 6, 2025

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
11:17

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

Published on: June 1, 2017

35.7K
Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis
05:47

Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis

Published on: June 25, 2020

5.3K
Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery
09:28

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery

Published on: October 27, 2023

3.2K

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Lipidomics

Background:

  • Untargeted lipidomics requires efficient lipid extraction for accurate analysis.
  • Liquid Chromatography High-Resolution Mass Spectrometry (LC-HRMS) is a powerful tool for lipid profiling.
  • Optimizing sample preparation is crucial for maximizing data quality and reproducibility.

Purpose of the Study:

  • To evaluate single-phase extraction methods for untargeted lipidomics.
  • To assess the efficacy of a novel tip-tip filtration technique for sample purification.
  • To improve the efficiency and cost-effectiveness of lipid extraction protocols.

Main Methods:

  • Evaluation of three single-phase lipid extraction solvents from pooled human plasma.
  • Analysis using Liquid Chromatography High-Resolution Mass Spectrometry (LC-HRMS) in positive and negative Electrospray Ionization (ESI).
  • Implementation and assessment of a "tip-tip filtration" method using a filtrating pipette tip for sample cleanup.

Main Results:

  • A methanol/chloroform (2:1, v/v) mixture provided the most satisfactory extraction results.
  • Tip-tip filtration significantly improved extract cleanliness and mass spectrometry compatibility compared to solvent extraction alone.
  • The filtration method enhanced reproducibility and maintained lipid coverage while reducing costs and time.

Conclusions:

  • The optimized methanol/chloroform extraction combined with tip-tip filtration is a superior method for untargeted lipidomics.
  • Tip-tip filtration offers a cost-effective and time-efficient approach to sample purification.
  • This method has the potential to increase the analytical column lifetime in LC-HRMS systems.