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Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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Related Experiment Video

Updated: Oct 6, 2025

Predicting Gene Silencing Through the Spatiotemporal Control of siRNA Release from Photo-responsive Polymeric Nanocarriers
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Two-Photon-Induced Charge-Variable Conjugated Polyelectrolyte Brushes for Effective Gene Silencing.

Hui Zhao1, Haojie Tao1, Wenbo Hu2

  • 1Key Laboratory for Organic Electronics and Information Displays & Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts & Telecommunications, 9 Wenyuan Road, Nanjing 210023, China.

ACS Applied Bio Materials
|January 14, 2022
PubMed
Summary

This study introduces a novel cationic conjugated polyelectrolyte brush for enhanced small interfering RNA (siRNA) delivery. The material enables efficient siRNA loading and controlled release via two-photon excitation, improving gene transfection efficiency.

Keywords:
charge-variableconjugated polyelectrolyte brushsiRNA therapeuticstwo-photon absorption

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Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
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Area of Science:

  • Biomaterials Science
  • Gene Delivery Systems
  • Polymer Chemistry

Background:

  • Cationic conjugated polyelectrolytes are explored as siRNA carriers but suffer from low charge density and poor intracellular release.
  • Efficient gene transfection requires high siRNA carrying capability and controlled intracellular release.

Purpose of the Study:

  • To develop a novel two-photon-induced charge-variable conjugated polyelectrolyte brush for efficient siRNA delivery.
  • To overcome the limitations of traditional siRNA carriers regarding carrying capacity and controlled release.

Main Methods:

  • Synthesis of a cationic conjugated polyelectrolyte brush (PPENBr-ONB) with densely cationic charges and a photoresponsive side chain.
  • Characterization of siRNA loading capacity using agarose gel electrophoresis.
  • Evaluation of two-photon absorption (TPA) cross-section and siRNA release under 720 nm illumination.
  • In vitro assessment of gene silencing efficacy targeting Plk1 mRNA.

Main Results:

  • PPENBr-ONB demonstrated remarkable siRNA carrying capability (1 mol PPENBr-ONB to >20 mol siRNA).
  • The polyelectrolyte brush exhibited a large TPA cross-section (1.47 × 10^5 GM), facilitating efficient two-photon-induced photolysis and siRNA release (78% at 720 nm).
  • In vitro experiments showed effective Plk1 mRNA knockdown (24.7%) using the PPENBr-ONB/siRNA complex under 720 nm illumination.

Conclusions:

  • The developed two-photon-induced charge-variable conjugated polyelectrolyte brush is an efficient siRNA carrier.
  • This system offers a promising strategy for photo-controlled gene delivery, overcoming limitations of existing siRNA therapeutics.