Male and female developmental temperature modulate post-copulatory interactions in a beetle

Sexual selection theory has proven to be fundamental to our understanding of the male-female (sperm-egg) interactions that characterise fertilisation. However, sexual selection does not operate in a void and abiotic environmental factors have been shown to modulate the outcome of pre-copularory sexual interactions. Environmental modulation of post-copulatory interactions are particularly likely because the form and function of primary reproductive traits appears to be acutely sensitive to temperature stress. Here we report the effects of developmental temperature on female reproductive architecture and the interaction between male and female developmental temperature on the outcome of sperm competition in the bruchid beetle Callosobruchus maculatus. When females were reared at developmental temperatures above and below typical temperatures the bursa copulatrix (site of spermatophore deposition) were smaller and, were either shorter and broader (high temperatures) or longer and thinner (low temperatures) than those reared at intermediate temperatures. Males and females reared at low developmental temperatures were less likely to mate than those reared at higher temperatures. Where copulation occurred, females reared at the highest temperature copulated for longest, whilst males reared at the lowest temperature spent longer in copula. Male developmental temperature had a significant impact on the outcome of sperm competition: males reared at 17 °C were largely unsuccessful in sperm competition against control (27 °C) males, although some of the variation in the outcome of sperm competition was a product of the interaction between male and female developmental temperature. Our results demonstrate that male-female interactions that characterise pre- and post-copulatory outcomes are sensitive to developmental temperature and that plasticity in cryptic female preferences could lead to heterogeneous selection on the male reproductive phenotype.