Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

3.7K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
3.7K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

9.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
9.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<math><mrow><mi>Micro</mi> <mi>S</mi> <mi>plit</mi></mrow></math> : semantic unmixing of fluorescent microscopy data.

Nature methods·2026
Same author

Molecular architecture of the ciliary base in mammalian multiciliated cells.

bioRxiv : the preprint server for biology·2026
Same author

HAK-actin, a U-ExM-compatible probe to image the actin cytoskeleton.

Cell reports methods·2026
Same author

An Interview With Marine Laporte, Winner of the SBCF Young Researcher Prize, 2025.

Biology of the cell·2026
Same author

Expansion Microscopy: High-Resolution Fluorescent Imaging with a Conventional Microscope.

Journal of visualized experiments : JoVE·2026
Same author

The luminal ring protein C2CD3 acts as a radial in-to-out organizer of the distal centriole and appendages.

PLoS biology·2025

Related Experiment Video

Updated: Oct 6, 2025

Visualization of Organelles In Situ by Cryo-STEM Tomography
08:37

Visualization of Organelles In Situ by Cryo-STEM Tomography

Published on: June 23, 2023

2.5K

Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM).

Marine H Laporte1, Nikolai Klena1, Virginie Hamel2

  • 1Department of Cell Biology, University of Geneva, Geneva, Switzerland.

Nature Methods
|January 14, 2022
PubMed
Summary

Cryofixation preserves cell structure better than chemical fixation but is challenging to implement. We developed Cryo-ExM, a simpler method combining cryofixation and expansion microscopy for improved super-resolution imaging.

More Related Videos

Visualizing Intracellular Sialylation with Click Chemistry and Expansion Microscopy
08:16

Visualizing Intracellular Sialylation with Click Chemistry and Expansion Microscopy

Published on: February 7, 2025

641
Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells
11:55

Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells

Published on: May 28, 2021

4.3K

Related Experiment Videos

Last Updated: Oct 6, 2025

Visualization of Organelles In Situ by Cryo-STEM Tomography
08:37

Visualization of Organelles In Situ by Cryo-STEM Tomography

Published on: June 23, 2023

2.5K
Visualizing Intracellular Sialylation with Click Chemistry and Expansion Microscopy
08:16

Visualizing Intracellular Sialylation with Click Chemistry and Expansion Microscopy

Published on: February 7, 2025

641
Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells
11:55

Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells

Published on: May 28, 2021

4.3K

Area of Science:

  • Cell biology
  • Microscopy techniques
  • Biophysical imaging

Background:

  • Chemical fixation is standard for microscopy but introduces artifacts.
  • Cryofixation offers superior preservation of native cellular ultrastructure.
  • Implementing cryofixation in fluorescence microscopy is challenging.

Purpose of the Study:

  • To develop a method combining cryofixation with expansion microscopy.
  • To overcome implementation challenges of cryofixation in fluorescence microscopy.
  • To enable high-resolution imaging of native cellular structures.

Main Methods:

  • Coupling cryofixation with expansion microscopy (ExM).
  • Developing Cryo-ExM for simplified cryofixation workflow.
  • Utilizing expansion microscopy for enhanced resolution.

Main Results:

  • Cryo-ExM successfully preserves native cellular organization.
  • The method bypasses artifacts typically associated with chemical fixation.
  • Demonstrated potential for widespread use in super-resolution microscopy.

Conclusions:

  • Cryo-ExM offers an accessible approach to high-fidelity cellular imaging.
  • This technique enhances the preservation of native cell ultrastructure.
  • Cryo-ExM is poised to advance super-resolution microscopy applications.