Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM)
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Updated: Oct 6, 2025

Visualization of Organelles In Situ by Cryo-STEM Tomography
Published on: June 23, 2023
Marine H Laporte1, Nikolai Klena1, Virginie Hamel2
1Department of Cell Biology, University of Geneva, Geneva, Switzerland.
Abstract:
Cryofixation has proven to be the gold standard for efficient preservation of native cell ultrastructure compared to chemical fixation, but this approach is not widely used in fluorescence microscopy owing to implementation challenges. Here, we develop Cryo-ExM, a method that preserves native cellular organization by coupling cryofixation with expansion microscopy. This method bypasses artifacts associated with chemical fixation and its simplicity will contribute to its widespread use in super-resolution microscopy.
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