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A quantitative detection of mung bean in chestnut paste using duplex digital PCR
Yingjie Liang1, Dongwei Gao1, Jie Dong1
1Guangzhou Customs Technology Center, No. 66 Huacheng Avenue, Tianhe District, Guangzhou, China.
Current Research in Food Science
|January 14, 2022
Summary
Economically Motivated Adulteration (EMA) of chestnut paste with mung bean can be detected using a novel duplex digital PCR method. This technique accurately quantifies mung bean content from 5% to 80%, ensuring product authenticity.
Area of Science:
- Food Science
- Analytical Chemistry
- Genetics
Background:
- Chestnut paste is susceptible to Economically Motivated Adulteration (EMA) with cheaper ingredients like mung bean.
- Current detection methods may lack the specificity and quantification accuracy needed to identify adulteration.
Purpose of the Study:
- To develop a novel quantitative detection method for mung bean in chestnut paste.
- To establish a reliable technique for identifying fraudulent substitution and adventitious contamination.
Main Methods:
- Development of a duplex digital PCR (ddPCR and cdPCR) assay using specific primers and probes for mung bean and chestnut genomic DNA.
- Establishment of a quantitative detection method based on the gene copy number (CN) ratio correlated with the mass ratio of ingredients.
- Determination of limits of quantification (LOQcopy) for both target species.
Main Results:
- The duplex digital PCR method accurately quantified mung bean in chestnut paste within a mass ratio range of 5% to 80%.
- The limits of quantification for gene CN concentrations (LOQcopy) for both mung bean and chestnut were determined to be 6 copies/μL.
- A linear relationship was established between the mass ratio and the gene copy number ratio.
Conclusions:
- Duplex digital PCR offers a precise and accurate method for quantifying mung bean adulteration in chestnut paste.
- This technique provides crucial technical support for food quality control and authenticity verification in chestnut paste products.
