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Evidence for arrested G2 cell subpopulation in rat liver inducible to mitosis
1Centro de Investigación, Hospital La Fe, Valencia, Spain.
Abstract:
The intraperitoneal administration of several substances (biliverdin, heat-killed bacteria and diatomaceous earth) to rats caused the prompt appearance of a mitotic wave in the liver. Autoradiographic analysis of livers of treated animals showed no evidence of [3H]-thymidine uptake by mitotic hepatocytes. In addition, livers from xenobiotic-treated rats showed a very low thymidine kinase activity, close to that found in normal livers. This excludes the possibility that non-cycling cells move to mitosis through the S phase. The results suggest that mitosis could be derived from a hepatocyte subpopulation arrested in the G2 phase of the cell cycle, which is stimulated to divide by the xenobiotics.
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