Related Experiment Video
Updated: Oct 6, 2025

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
Published on: March 30, 2018
A unique miR775-GALT9 module regulates leaf senescence in Arabidopsis during post-submergence recovery by modulating
Vishnu Mishra1, Archita Singh1,2, Nidhi Gandhi1
1National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India.
Abstract:
The submergence-induced hypoxic condition negatively affects the plant growth and development, and causes early onset of senescence. Hypoxia alters the expression of a number of microRNAs (miRNAs). However, the molecular function of submergence stress-induced miRNAs in physiological or developmental changes and recovery remains poorly understood. Here, we show that miR775 is an Arabidopsis thaliana-specific young and unique miRNA that possibly evolved non-canonically. miR775 post-transcriptionally regulates GALACTOSYLTRANSFERASE 9 (GALT9) and their expression is inversely affected at 24 h of complete submergence stress. The overexpression of miR775 (miR775-Oe) confers enhanced recovery from submergence stress and reduced accumulation of RBOHD and ROS, in contrast to wild-type and MIM775 Arabidopsis shoot. A similar recovery phenotype in the galt9 mutant indicates the role of the miR775-GALT9 module in post-submergence recovery. We predicted that Golgi-localized GALT9 is potentially involved in protein glycosylation. The altered expression of senescence-associated genes (SAG12, SAG29 and ORE1), ethylene signalling (EIN2 and EIN3) and abscisic acid (ABA) biosynthesis (NCED3) pathway genes occurs in miR775-Oe, galt9 and MIM775 plants. Thus, our results indicate the role for the miR775-GALT9 module in post-submergence recovery through a crosstalk between the ethylene signalling and ABA biosynthesis pathways.
Related Concept Videos
Adaptations that Reduce Water Loss
Regulation of Transpiration by Stomata
Cell Signaling in Plants
C4 Pathway and CAM
C4 Pathway
The C4 pathway is used by plants such as...
Responses to Salt Stress
Responses to Drought and Flooding

