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Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis

Guang Shan1, Ting Huang2, Tian Tang3

  • 1Department of Urology, Renmin Hospital of Wuhan University, Wuhan, Hubei, China.

Pathology, Research and Practice
|January 14, 2022
PubMed

Insights

Long non-coding RNA MEG8 is upregulated in clear cell renal cell carcinoma (ccRCC). Its inhibition suppresses ccRCC progression, revealing a PLAG1/MEG8/miR-495-3p/G3BP1 network for potential ccRCC diagnostics and therapeutics.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Clear cell renal cell carcinoma (ccRCC) is a lethal malignancy with increasing mortality.
  • The molecular mechanisms of ccRCC progression are not fully understood.
  • Long non-coding RNAs (lncRNAs) play significant roles in various diseases.

Purpose of the Study:

  • To investigate the role of lncRNA MEG8 in clear cell renal cell carcinoma (ccRCC) development.
  • To identify the regulatory network involving MEG8 in ccRCC.

Main Methods:

  • Analysis of MEG8 expression in ccRCC tissues and cells.
  • In vitro and in vivo experiments to assess the functional impact of MEG8 knockdown.
  • Bioinformatics analysis, ChIP, and luciferase assays to determine regulatory relationships.
  • Investigation of the interaction between MEG8, miR-495-3p, and G3BP1.

Main Results:

  • MEG8 expression is significantly increased in ccRCC tissues and cells.
  • Knockdown of MEG8 inhibited ccRCC cell viability, migration, invasion, and tumor growth.
  • PLAG1 was identified as a transcriptional regulator of MEG8.
  • MEG8 acts as a sponge for miR-495-3p, leading to increased G3BP1 expression and promotion of ccRCC tumorigenesis.

Conclusions:

  • A novel regulatory network (PLAG1/MEG8/miR-495-3p/G3BP1) was identified in ccRCC development.
  • This network represents a potential target for new diagnostic or therapeutic strategies for ccRCC.
Keywords:
Clear cell renal cell carcinomaG3BP1LncRNA MEG8MiR-495–3pPLAG1

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