Medium-chain carboxylates production by co-fermentation of sewage sludge and macroalgae

The co-fermentation of sewage sludge and macroalgae at different mixing ratios was performed for medium-chain carboxylates (MCCs) production. The results showed that MCCs production was enhanced in co-fermentation groups due to the abundant readily available organics supplied by macroalgae and the alkaline buffer capacity provided by sewage sludge. Highest MCCs concentration of 112.7 mmol C/L (25.5 mmol C/g VS_added) was obtained in the co-fermentation group with sludge/macroalgae ratio of 4:6, which was higher than MCCs produced from the mono-fermentation of sewage sludge (41.7 mmol C/L, 9.4 mmol C/g VS_added) or macroalgae (79.9 mmol C/L, 18.1 mmol C/g VS_added). Microbial analysis showed that species from genus Romboutsia, Terrisporobacter, Clostridium_sensu_stricto_12, Paraclostridium, unclassified_f_Peptostreptococcaceae and Caproiciproducens were significantly positively correlated with MCCs production. Metabolic pathways analysis demonstrated that the co-fermentation promoted the chain elongation process by stimulating the rate-limiting steps involved in the conversion of ethanol to Acetyl-CoA and circular fatty acid biosynthesis pathway.