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Proteomics based markers of clinical pain severity in juvenile idiopathic arthritis

Hanne Van Der Heijden1,2,3, Benoit Fatou4, Diana Sibai1

  • 1Department of Anesthesiology, Critical Care and Pain Medicine, Boston Children's Hospital, Harvard Medical School, Boston, MA, USA.

Pediatric Rheumatology Online Journal
|January 16, 2022
PubMed

Insights

This pilot study explored serum proteomes in children with juvenile idiopathic arthritis (JIA) to understand pain mechanisms. Key proteins linked to pain severity and inflammation were identified, suggesting both inflammatory and non-inflammatory drivers of JIA pain.

Area of Science:

  • Proteomics
  • Rheumatology
  • Pediatric Autoimmune Diseases

Background:

  • Juvenile idiopathic arthritis (JIA) is a group of autoimmune rheumatic diseases affecting children.
  • The molecular mechanisms underlying pain in JIA remain largely unclear.
  • This study investigated serum proteome variability in relation to pain severity in JIA patients.

Purpose of the Study:

  • To explore the serum proteome in JIA patients.
  • To identify proteins associated with pain severity in JIA.
  • To investigate correlations between pain-associated proteins, inflammation markers, and CNS morphology.

Main Methods:

  • Serum samples from 15 JIA patients were analyzed using liquid chromatography/mass spectrometry (LC/MS).
  • Correlation analyses assessed relationships between protein levels and self-reported pain severity.
  • Associations with Erythrocyte Sedimentation Rate (ESR), subcortical volume, and cortical thickness were evaluated.

Main Results:

  • 306 proteins were identified in the JIA cohort.
  • 14 proteins showed a significant association with clinical pain severity (p < 0.05).
  • Pain-associated proteins were linked to humoral immunity, inflammatory response, angiogenesis, ESR, and CNS morphology.

Conclusions:

  • Proteomic findings suggest both inflammatory and non-inflammatory mechanisms contribute to JIA pain.
  • Preliminary observations indicate potential novel serologic markers for JIA pain.
  • Further validation in larger cohorts and longitudinal studies is recommended.
Abstract

Keywords:
InflammationJuvenile idiopathic arthritisMass spectrometryPainProteomics

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