Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

14.9K
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
14.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Wax-printing-free fabrication of paper-supported 3D cancer cell culture.

Analytical methods : advancing methods and applications·2026
Same author

Retraction notice to "Experimental investigations of dual functional substrate integrated waveguide antenna with enhanced directivity for 5G mobile communications" [Heliyon 10 (2024) e36929].

Heliyon·2026
Same author

6G -enabled qubit-based concealed communication with AI-driven breach detection in autonomous fleets.

Scientific reports·2025
Same author

Advanced finite segmentation model with hybrid classifier learning for high-precision brain tumor delineation in PET imaging.

Scientific reports·2025
Same author

Cross-correlation-based signal pruning method (CCSPM) for effective signal distortion reduction in massive MIMO communications.

Scientific reports·2025
Same author

Laser melting of Parafilm-wax: a novel approach to hydrophobic barrier formation in paper-based microfluidics.

Analytical methods : advancing methods and applications·2025

Related Experiment Video

Updated: Oct 6, 2025

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays
11:33

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Published on: March 9, 2017

15.9K

Paper-microfluidic signal-enhanced immunoassays.

N Sathishkumar1, Bhushan J Toley1

  • 1Department of Chemical Engineering, Indian Institute of Science, Bengaluru, KA, India.

Progress in Molecular Biology and Translational Science
|January 16, 2022
PubMed
Summary

Paper-based microfluidic devices offer low-cost diagnostics. Researchers are developing strategies to improve the sensitivity of lateral flow immunoassays (LFIAs) for better bedside testing applications.

Keywords:
ELISAImmunoassayLateral flow assayMicroPADsPaper-based microfluidic devicesPoint-of-care diagnosisSensitivitySignal enhancement

More Related Videos

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays
09:58

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Published on: June 23, 2022

2.3K
Author Spotlight: Revolutionizing Microfluidics Through Microchannel Fabrication on Nanopaper
03:58

Author Spotlight: Revolutionizing Microfluidics Through Microchannel Fabrication on Nanopaper

Published on: October 6, 2023

1.9K

Related Experiment Videos

Last Updated: Oct 6, 2025

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays
11:33

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Published on: March 9, 2017

15.9K
Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays
09:58

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Published on: June 23, 2022

2.3K
Author Spotlight: Revolutionizing Microfluidics Through Microchannel Fabrication on Nanopaper
03:58

Author Spotlight: Revolutionizing Microfluidics Through Microchannel Fabrication on Nanopaper

Published on: October 6, 2023

1.9K

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Point-of-Care Diagnostics

Background:

  • Paper-based microfluidic devices are increasingly popular for simple, low-cost diagnostic tests.
  • Immunoassays, particularly lateral flow immunoassays (LFIA), are a key application, with home pregnancy tests being a prime example.
  • Traditional LFIAs exhibit lower analytical sensitivity compared to laboratory-based enzyme-linked immunoassays (ELISA), limiting their use in bedside diagnostics.

Purpose of the Study:

  • To review and present various strategies developed by researchers to enhance the sensitivity of membrane-based immunoassays.
  • To provide a comprehensive overview of advancements in improving LFIA sensitivity.
  • To guide future research directions through a SWOT analysis.

Main Methods:

  • Review of existing literature and research on sensitivity enhancement techniques for membrane-based immunoassays.
  • Categorization and presentation of different strategies employed to boost assay sensitivity.
  • Inclusion of a SWOT (Strengths, Weaknesses, Opportunities, Threats) analysis.

Main Results:

  • Several innovative strategies have been identified and presented for enhancing the sensitivity of LFIAs.
  • The chapter details various approaches to overcome the sensitivity limitations of traditional LFIAs.
  • A SWOT analysis is provided to assess the landscape and future potential of these enhancement strategies.

Conclusions:

  • Enhancing the sensitivity of membrane-based immunoassays is crucial for expanding the utility of paper-based diagnostic devices.
  • Continued research into novel strategies promises to bridge the gap between LFIA sensitivity and laboratory techniques like ELISA.
  • The presented strategies and SWOT analysis offer valuable insights for the future development of sensitive and accessible point-of-care diagnostic tools.