Related Experiment Video

Updated: Oct 6, 2025

Author Spotlight: RNA FISH for Locating lncRNA-SNHG6 in Osteosarcoma Cells
05:27

Author Spotlight: RNA FISH for Locating lncRNA-SNHG6 in Osteosarcoma Cells

Published on: June 16, 2023

1.8K

Silencing long non-coding RNA LINC00960 inhibits osteosarcoma proliferation by sponging miR-107 to downregulate SALL4

Yubo Shi1, Bo Yang1, Yingchun Zhao1

  • 1Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, 430060, China.

Biochemical and Biophysical Research Communications
|January 16, 2022
PubMed

Insights

Long non-coding RNA LINC00960 acts as an oncogene in osteosarcoma (OS) by promoting cell proliferation and tumor growth. It regulates SALL4 via sponging miR-107, suggesting LINC00960 as a potential therapeutic target for OS.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) are implicated in tumorigenesis, acting as either tumor suppressors or oncogenes.
  • Osteosarcoma (OS) is a primary bone malignancy with significant impact on patient prognosis.

Purpose of the Study:

  • To investigate the role and underlying mechanisms of LINC00960 in osteosarcoma progression.
  • To determine the potential of LINC00960 as a therapeutic target for OS.

Main Methods:

  • In vitro studies involving cell proliferation, migration, and invasion assays in OS cell lines (143B and MG63) with LINC00960 silencing.
  • In vivo studies using xenograft models to assess the effect of LINC00960 knockdown on tumor growth.
  • Mechanistic studies exploring the interaction between LINC00960, miR-107, and SALL4.

Main Results:

  • Silencing LINC00960 significantly inhibited proliferation, migration, and invasion of osteosarcoma cells in vitro.
  • Knockdown of LINC00960 repressed tumor growth in vivo.
  • LINC00960 was found to regulate SALL4 expression by sponging miR-107, thereby promoting OS progression.

Conclusions:

  • LINC00960 functions as an oncogene in the development and prognosis of osteosarcoma.
  • Targeting LINC00960 presents a potential novel therapeutic strategy for osteosarcoma treatment.
Keywords:
LINC00960OsteosarcomaSALL4miR-107

More Related Videos

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells09:45

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

8.5K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.2K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.0K
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.3K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
17.2K
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.6K