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'Cookies on a tray': Superselective hierarchical microstructured poly(l-lactide) surface as a decoy for cells
Bartłomiej Kryszak1, Konrad Szustakiewicz1, Paulina Dzienny2
1Department of Polymer Engineering and Technology, Faculty of Chemistry, Wrocław University of Science and Technology (WUST), Wyb. Wyspiańskiego 27, 50-370 Wrocław, Poland.
Biomaterials Advances
|January 17, 2022
Summary
Researchers created micro-sized hierarchical structures on poly(l-lactide) surfaces using femtosecond laser modification. This textured material enhances selective cell adhesion for potential use in smart implants and regenerative medicine.
Area of Science:
- Biomaterials Engineering
- Surface Science
- Regenerative Medicine
Background:
- Poly(l-lactide) (PLLA) is a biocompatible polymer often used in medical applications.
- Surface topography significantly influences cellular behavior and material interactions.
- Developing advanced surface structures is crucial for improving implant performance and tissue regeneration.
Purpose of the Study:
- To engineer micro-sized hierarchical structures on PLLA surfaces.
- To investigate the impact of these structures on cell adhesion and growth.
- To assess the potential of laser-modified PLLA for selective cell patterning and biomedical applications.
Main Methods:
- PLLA films were fabricated using casting melt extrusion.
- Femtosecond laser irradiation (λ = 1030 nm) was employed for surface texturing.
- Cellular responses of fibroblast, osteoblast, Staphylococcus aureus, and Saccharomyces boulardii were evaluated.
Main Results:
- Hierarchical microstructures resembling 'cookies on a tray' were successfully created.
- Laser-modified PLLA surfaces demonstrated enhanced adhesion and growth of fibro- and osteoblasts.
- Selective patterning of eukaryotic cells was achieved, while bacterial adhesion was non-preferential.
- Fungal adhesion patterns mirrored those of mammalian cells, differing from bacterial adhesion.
Conclusions:
- Femtosecond laser structuring of PLLA creates a non-cytotoxic, cell-adhesive surface.
- The developed hierarchical structures enable highly selective patterning of living eukaryotic cells.
- This technology holds promise for fabricating personalized and smart implants in regenerative medicine.
