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Updated: Oct 6, 2025
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Decellularization and Recellularization of Whole Livers
Published on: February 4, 2011
22.2K
Improving hemocompatibility of decellularized liver scaffold using Custodiol solution.
Marlon Lemos Dias1, Bruno Andrade Paranhos1, Juliana Ribeiro Pinheiro Ferreira2
1Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brasil; Instituto Nacional de Ciência e Tecnologia em Medicina Regenerativa, INCT-REGENERA, Universidade Federal do Rio de Janeiro, UFRJ, Rio de Janeiro, Brasil.
Biomaterials Advances
|January 17, 2022
Summary
Custodiol (HTK solution) enhances organ scaffold hemocompatibility by inhibiting blood coagulation. This tissue engineering strategy improves decellularized biomaterial safety for whole-organ transplantation.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Transplantation Biology
Background:
- Organ decellularization shows promise for addressing organ transplant shortages.
- A major challenge is the poor hemocompatibility of decellularized scaffolds.
- Existing acellular materials often trigger adverse blood reactions.
Purpose of the Study:
- To evaluate Custodiol (HTK solution) as a method to improve scaffold hemocompatibility.
- To assess the anti-thrombogenic effects of Custodiol on decellularized liver scaffolds.
- To determine the potential of Custodiol for enhancing whole-organ tissue engineering applications.
Main Methods:
- Decellularized rat liver scaffolds were perfused with Custodiol (HTK solution).
- Hemocompatibility was assessed through ex vivo, in vitro, and in vivo coagulation assays.
- Platelet aggregation, cellular compatibility, and proteomic analyses were performed.
Main Results:
- Custodiol perfusion significantly inhibited blood coagulation and platelet aggregation.
- Successful transplantation of whole-liver scaffolds into recipient animals was achieved.
- Proteomic analysis showed reduced thrombogenicity-related proteins in Custodiol-treated scaffolds.
Conclusions:
- Custodiol (HTK solution) effectively enhances the hemocompatibility of decellularized scaffolds.
- This perfusion strategy reduces thrombogenicity, enabling successful whole-organ scaffold transplantation.
- Custodiol offers a promising approach for advancing whole-organ tissue engineering.
