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Updated: Oct 6, 2025

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
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Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

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Role of miR-100-5p and CDC25A in breast carcinoma cells

Xiaoping Li1,2, Yanli Ren3, Donghong Liu4

  • 1Faculty of Medicine, Macau University of Science and Technology, Macau, China.

Peerj
|January 17, 2022
PubMed
Abstract

Insights

MicroRNA-100-5p (miR-100-5p) is downregulated in breast cancer (BC), while CDC25A is upregulated. Restoring miR-100-5p levels inhibits BC progression and may serve as a diagnostic marker.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Breast carcinoma (BC) is a significant global health concern.
  • Understanding the molecular mechanisms driving BC progression is crucial for developing effective targeted therapies.

Purpose of the Study:

  • To investigate the role of the miR-100-5p/CDC25A axis in breast carcinoma.
  • To explore the potential of this axis as a therapeutic target for BC.

Main Methods:

  • Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) to measure miR-100-5p and CDC25A mRNA levels.
  • Western blot analysis to assess CDC25A protein expression.
  • Dual-luciferase reporter assay to confirm the targeting relationship between miR-100-5p and CDC25A.
  • In vitro cell function assays to evaluate the impact on BC cell proliferation, migration, invasion, and apoptosis.

Main Results:

  • miR-100-5p was significantly downregulated in BC tissues and cells, whereas CDC25A was highly expressed.
  • miR-100-5p directly downregulated CDC25A expression.
  • Forced expression of miR-100-5p inhibited BC cell proliferation, migration, and invasion, while promoting apoptosis.
  • Overexpression of miR-100-5p counteracted the promoting effects of CDC25A on BC cell growth.
  • miR-100-5p expression levels correlated with patient prognosis.

Conclusions:

  • CDC25A is identified as a key target of miR-100-5p in mediating BC progression.
  • miR-100-5p overexpression or CDC25A suppression shows potential for BC diagnosis and targeted therapy.
Keywords:
Breast carcinomaCDC25AMiR-100-5pMigration and invasionProliferation

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