Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

22.1K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
22.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Protective Role of Rosmarinic Acid-Enriched <i>Elsholtzia kachinensis</i> Extract Against Oxidative and Inflammatory Stress in Human Trophoblasts Through the miR-138-5p/FOXC1 Pathway.

Biochemistry research international·2026
Same author

Renoprotective Potential of Beetroot Spent Extract Under Hyperglycemic Conditions.

Foods (Basel, Switzerland)·2026
Same author

Green Tea Extract Containing Epigallocatechin-3-Gallate Facilitates Bone Formation and Mineralization by Alleviating Iron-Overload-Induced Oxidative Stress in Human Osteoblast-like (MG-63) Cells.

Antioxidants (Basel, Switzerland)·2025
Same author

Phytochemical Screening and <i>In Vitro</i> Antioxidant and Anticancer Evaluation of Stem and Leaf Extracts of <i>Cissampelos pareira</i> L.

Mediators of inflammation·2025
Same author

Assessing the Antioxidant, Hepatoprotective, and Iron-Chelating Potential of <i>Perilla frutescens</i> Seed.

Biomedicines·2025
Same author

How can we reduce maternal mortality due to preeclampsia? The 4P rule.

Revista brasileira de ginecologia e obstetricia : revista da Federacao Brasileira das Sociedades de Ginecologia e Obstetricia·2024

Related Experiment Video

Updated: Oct 6, 2025

Human Primary Trophoblast Cell Culture Model to Study the Protective Effects of Melatonin Against Hypoxia/reoxygenation-induced Disruption
12:02

Human Primary Trophoblast Cell Culture Model to Study the Protective Effects of Melatonin Against Hypoxia/reoxygenation-induced Disruption

Published on: July 30, 2016

16.1K

PM10 Alters Trophoblast Cell Function and Modulates miR-125b-5p Expression.

Wittaya Chaiwangyen1, Komsak Pintha1, Payungsak Tantipaiboonwong1

  • 1Division of Biochemistry, School of Medical Sciences, University of Phayao, Phayao 56000, Thailand.

Biomed Research International
|January 17, 2022
PubMed
Summary

Air pollution, specifically Particulate Matter 10 (PM10), harms trophoblast cells crucial for pregnancy. PM10 exposure dysregulates miR-125b-5p, impacting placental cell function and potentially leading to pregnancy complications.

More Related Videos

A Primary Human Trophoblast Model to Study the Effect of Inflammation Associated with Maternal Obesity on Regulation of Autophagy in the Placenta
11:44

A Primary Human Trophoblast Model to Study the Effect of Inflammation Associated with Maternal Obesity on Regulation of Autophagy in the Placenta

Published on: September 27, 2017

11.6K
Trophoblast Cell Recovery from Angiogenesis-Tube Formation Assay for Differentiation Marker Expression Analysis
05:30

Trophoblast Cell Recovery from Angiogenesis-Tube Formation Assay for Differentiation Marker Expression Analysis

Published on: November 8, 2024

623

Related Experiment Videos

Last Updated: Oct 6, 2025

Human Primary Trophoblast Cell Culture Model to Study the Protective Effects of Melatonin Against Hypoxia/reoxygenation-induced Disruption
12:02

Human Primary Trophoblast Cell Culture Model to Study the Protective Effects of Melatonin Against Hypoxia/reoxygenation-induced Disruption

Published on: July 30, 2016

16.1K
A Primary Human Trophoblast Model to Study the Effect of Inflammation Associated with Maternal Obesity on Regulation of Autophagy in the Placenta
11:44

A Primary Human Trophoblast Model to Study the Effect of Inflammation Associated with Maternal Obesity on Regulation of Autophagy in the Placenta

Published on: September 27, 2017

11.6K
Trophoblast Cell Recovery from Angiogenesis-Tube Formation Assay for Differentiation Marker Expression Analysis
05:30

Trophoblast Cell Recovery from Angiogenesis-Tube Formation Assay for Differentiation Marker Expression Analysis

Published on: November 8, 2024

623

Area of Science:

  • Environmental Health
  • Reproductive Biology
  • Molecular Toxicology

Background:

  • Air pollution, particularly Particulate Matter 10 (PM10), is a significant global health risk.
  • PM10 exposure is linked to various diseases through altered microRNA (miRNA) expression.
  • Trophoblast cells are vital for successful pregnancy, but PM10's effects on them remain unclear.

Purpose of the Study:

  • To investigate the impact of PM10 on human trophoblast cell function (HTR-8/SVneo).
  • To explore the role of miRNA expression in PM10-induced trophoblast dysfunction.
  • To identify specific molecular targets affected by PM10 in trophoblast cells.

Main Methods:

  • Exposure of HTR-8/SVneo trophoblast cells to varying concentrations of PM10.
  • Assessment of cell viability, proliferation, migration, invasion, and apoptosis.
  • Measurement of inflammatory cytokine (IL-1β, IL-6, TNF-α) and miRNA (miR-125b-5p) expression.
  • Bioinformatic analysis to identify potential miRNA targets (TXNRD1).

Main Results:

  • PM10 exposure reduced trophoblast cell viability, proliferation, migration, and invasion.
  • High PM10 concentrations induced significant trophoblast cell apoptosis.
  • PM10 upregulated inflammatory markers (IL-1β, IL-6, TNF-α) and miR-125b-5p expression.
  • miR-125b-5p was negatively correlated with its target gene, TXNRD1.

Conclusions:

  • PM10 exposure impairs critical trophoblast cell functions essential for pregnancy.
  • PM10 alters miR-125b-5p expression, targeting TXNRD1 and contributing to cellular dysfunction.
  • Further research on miR-125b-5p in PM10-exposed trophoblasts may yield biomarkers or therapies.