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Isoelectric focusing of human salivary secretory-IgA

R J Mairs1, J A Beeley

  • 1Oral Biochemistry Unit, University of Glasgow Dental School, Scotland, U.K.

Archives of Oral Biology
|January 1, 1987
PubMed

A method using overlays of 131I-labelled antisera to IgA or SC was developed for the specific detection of s-IgA in unpurified saliva after isoelectric focusing in polyacrylamide gels. The pI range of salivary s-IgA was 4.8-6.5; that of colostral s-IgA was slightly lower (4.5-5.5).

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Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
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