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Updated: Oct 6, 2025
Analyzing Platelet Subpopulations by Multi-color Flow Cytometry
Published on: June 10, 2025
Anti-GPVI (HY101) activates platelets in a multicolor flow cytometry panel
Marie-Astrid van Dievoet1, Davide Brusa2, Marie Octave3
1Laboratoire d'Hépatologie Pédiatrique et Thérapie Cellulaire, Unité Pedi, Institut de Recherche Expérimentale et Clinique (IREC), Université Catholique de Louvain (Uclouvain), Brussels, Belgium.
The platelet transmembrane receptor GPVI can be assessed together with other platelet membrane markers in a whole blood multicolor flow cytometry panel. The advantage of combining multiple antibodies in a single tube is the possibility of distinguishing multiple platelet subgroups. In this short communication, we describe an activation problem encountered with anti-GPVI, clone HY101. Activation of platelets was seen after the addition of anti-GPVI in a flow cytometry panel, highlighted by the expression of the activation markers CD62P, PAC-1, CD63, and CD107a. This was also confirmed by platelet aggregation studies.
The platelet transmembrane receptor GPVI can be assessed together with other platelet membrane markers in a whole blood multicolor flow cytometry panel. The advantage of combining multiple antibodies in a single tube is the possibility of distinguishing multiple platelet subgroups. In this short communication, we describe an activation problem encountered with anti-GPVI, clone HY101. Activation of platelets was seen after the addition of anti-GPVI in a flow cytometry panel, highlighted by the expression of the activation markers CD62P, PAC-1, CD63, and CD107a. This was also confirmed by platelet aggregation studies.
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