Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cross-reactivity

Cross-reactivity

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Cutaneous eruptions associated with human polyomavirus 6 and human polyomavirus 7 in liver transplant recipients: Two case reports.

JAAD case reports·2026

Same author

Towards the development of a mucosal vectored vaccine against enterovirus D68.

Virology·2026

Same author

BCS2.0: a capture sequencing platform for rapid differential diagnosis of bacterial infections and antimicrobial resistance.

Frontiers in microbiology·2026

Same author

Evaluation of immunoreactive epitopes in the sera and cerebrospinal fluid of patients with post-treatment Lyme disease syndrome.

Scientific reports·2026

Same author

Adaptation of custom capture sequencing panels to the Oxford Nanopore MinION platform.

Molecular biology reports·2026

Same author

Capture sequencing demonstrates promising public health potential for post-COVID wastewater surveillance: a comparative multi-technique and spatiotemporal analysis.

Environment international·2026

Search research articles

Related Experiment Video

Updated: Oct 6, 2025

Particle Agglutination Method for Poliovirus Identification

Particle Agglutination Method for Poliovirus Identification

Published on: April 20, 2011

Cross-Reactive Antibody Responses against Nonpoliovirus Enteroviruses.

Amy B Rosenfeld¹, Edmund Qian Long Shen¹, Michaela Melendez¹

¹Department of Microbiology and Immunology, Vagelos College of Physicians and Surgeons, Columbia Universitygrid.239585.0grid.21729.3f, New York, New York, USA.

|January 18, 2022

Summary

Enteroviruses (EVs) are common pathogens causing serious illnesses. Cross-reactive antibodies induced by EVs can bind and neutralize different types, potentially impacting disease severity and offering insights into EV infections.

Keywords:

antibody antigenic variation enterovirus poliovirus serotype

More Related Videos

Detection of Polyfunctional T Cells in Children Vaccinated with Japanese Encephalitis Vaccine via the Flow Cytometry Technique

Detection of Polyfunctional T Cells in Children Vaccinated with Japanese Encephalitis Vaccine via the Flow Cytometry Technique

Published on: September 23, 2022

A Liquid Phase Affinity Capture Assay Using Magnetic Beads to Study Protein-Protein Interaction: The Poliovirus-Nanobody Example

A Liquid Phase Affinity Capture Assay Using Magnetic Beads to Study Protein-Protein Interaction: The Poliovirus-Nanobody Example

Published on: May 29, 2012

Related Experiment Videos

Last Updated: Oct 6, 2025

Particle Agglutination Method for Poliovirus Identification

Particle Agglutination Method for Poliovirus Identification

Published on: April 20, 2011

Detection of Polyfunctional T Cells in Children Vaccinated with Japanese Encephalitis Vaccine via the Flow Cytometry Technique

Detection of Polyfunctional T Cells in Children Vaccinated with Japanese Encephalitis Vaccine via the Flow Cytometry Technique

Published on: September 23, 2022

A Liquid Phase Affinity Capture Assay Using Magnetic Beads to Study Protein-Protein Interaction: The Poliovirus-Nanobody Example

A Liquid Phase Affinity Capture Assay Using Magnetic Beads to Study Protein-Protein Interaction: The Poliovirus-Nanobody Example

Published on: May 29, 2012

Area of Science:

Virology
Immunology
Infectious Diseases

Background:

Enteroviruses (EVs) are frequent human pathogens responsible for diverse illnesses, including acute flaccid myelitis and hand-foot-mouth disease.
Despite genetic diversity, nonpoliovirus enteroviruses (NPEVs) exhibit antigenic similarities, leading to cross-reactive antibodies capable of binding or neutralizing multiple EV types.

Purpose of the Study:

To investigate the antigenic relationships among various enteroviruses, including poliovirus and NPEVs.
To determine if cross-reactive antibodies can bind and neutralize heterologous EVs, and explore their potential role in modulating infection and disease.

Main Methods:

Utilized plaque reduction and enzyme-linked immunosorbent assay (ELISA)-based binding assays.
Analyzed sera from animals immunized with individual EVs to assess binding and neutralization activities against heterologous EVs.

Main Results:

Demonstrated extensive cross-reactivity among enteroviruses, which was not predictable from phylogenetic analysis.
Observed both binding and neutralization activities of anti-EV cross-reactive antibodies against heterologous EVs in animal sera.

Conclusions:

The immunologic relationship among EVs is crucial for understanding humoral responses during EV infections.
Cross-reactive antibodies may play a role in modulating enterovirus infection and disease severity, offering insights into EV epidemiology and pathogenesis.