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Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Efficient SARS-CoV-2 Quantitative Reverse Transcriptase PCR Saliva Diagnostic Strategy utilizing Open-Source Pipetting Robots

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Exploring Workplace Testing with Real-Time Polymerase Chain Reaction SARS-CoV-2 Testing.

Lucas Fuentes¹, Nigam Shah¹, Sara Kelly¹

¹From Department of Electrical Engineering, Stanford University, Stanford, CA (LF); Department of Biomedical Informatics, Stanford University, Stanford, CA (NS); Clinical Excellence Research Center, Stanford University, Stanford, CA (SK); Department of Medicine, Stanford University, Stanford, CA (KS).

Journal of the American Board of Family Medicine : JABFM

|January 18, 2022

Summary

Point-of-care (POC) real-time polymerase chain reaction (RT-PCR) testing for SARS-CoV-2 significantly reduces turnaround time compared to traditional lab testing. This rapid molecular testing enhances workplace safety by providing timely results for COVID-19 detection.

Keywords:

COVID-19 Point-of-Care Testing Polymerase Chain Reaction SARS-CoV-2 Screening Workplace

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Published on: April 9, 2021

Area of Science:

Molecular diagnostics
Infectious disease surveillance
Public health

Background:

SARS-CoV-2 detection relies on molecular (RT-PCR) and antigen tests.
RT-PCR tests are accurate but have longer result times than antigen tests.
Workplace safety necessitates rapid and reliable SARS-CoV-2 testing.

Purpose of the Study:

To evaluate point-of-care (POC) RT-PCR testing for SARS-CoV-2.
To compare POC RT-PCR with standard send-out PCR testing.
To assess the utility of POC testing for workplace safety.

Main Methods:

A program implemented in summer 2020 compared POC RT-PCR and send-out PCR tests.
Key metrics included test results and time-to-results.
Validation analysis was performed on a subset of samples.

Main Results:

POC testing reduced time to results from 754 minutes to 110 minutes.
Specificity of POC RT-PCR was 98.7% (99.8% in validation).
Sensitivity of POC testing was 100% in initial analysis, with clarification on validation findings.

Conclusions:

Point-of-care RT-PCR testing for SARS-CoV-2 is feasible.
POC testing offers a significant reduction in turnaround time compared to send-out PCR.
Rapid molecular diagnostics can support workplace safety initiatives.