Isolation and Characterization of Antimicrobial Peptides Isolated from Fagonia bruguieri
Khamis Sulaiman Al-Dhafri1,2, Chai Lay Ching3
1Department of Genetics and Microbiology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia. aldhafri2013@siswa.um.edu.my.
Abstract:
The majority of pathogenic microorganisms have developed resistance to commercial antibiotics. It causes the risk of illness relapse with current antimicrobial therapy regimens; additional and/or different antibacterial drugs are needed to treat diseases caused by these pathogenic microorganisms. The applied analysis in the present study was purification and characterization of plant peptides isolated from the leaves of Fagonia bruguieri as well as their antibacterial activities against Gram-positive bacteria, Staphylococcus aureus, Bacillus subtilis, Micrococcus luteus, and methicillin-resistant Staphylococcus aureus, in addition to Gram-negative bacteria, Escherichia coli and Pseudomonas aeruginosa. The minimum inhibitory concentration for the isolated peptide ranges from 25 to 62.5 mg/mL. The methanolic solvent was used for the extraction followed by reversed-phase high-performance liquid chromatography for purification of peptides. Eventually, the peptide characterization and identification were also determined by MALDI-TOF/TOF and SEM analysis. This study paves a way to the effective antimicrobials from the plant resources.
Insights
Plant-derived peptides from Fagonia bruguieri show promising antibacterial activity against resistant pathogens. This research highlights potential new antimicrobial drugs to combat antibiotic resistance.
Area of Science:
- Natural Product Chemistry
- Microbiology
- Pharmacology
Background:
- Antibiotic resistance in pathogenic microorganisms poses a significant global health threat.
- Current antimicrobial therapies are becoming less effective, necessitating the development of novel antibacterial agents.
- Plant-derived compounds are a promising source for discovering new antimicrobial drugs.
Purpose of the Study:
- To isolate and characterize peptides from Fagonia bruguieri leaves.
- To evaluate the antibacterial activity of these peptides against various Gram-positive and Gram-negative bacteria.
- To explore Fagonia bruguieri as a source of novel antimicrobial compounds.
Main Methods:
- Peptide extraction using methanolic solvent.
- Purification of peptides via reversed-phase high-performance liquid chromatography (RP-HPLC).
- Characterization and identification using MALDI-TOF/TOF and Scanning Electron Microscopy (SEM) analysis.
Main Results:
- Isolated peptides from Fagonia bruguieri demonstrated antibacterial activity against Staphylococcus aureus, Bacillus subtilis, Micrococcus luteus, methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli, and Pseudomonas aeruginosa.
- The minimum inhibitory concentration (MIC) of the isolated peptides ranged from 25 to 62.5 mg/mL.
- Successful purification and characterization of the plant peptides were achieved.
Conclusions:
- Fagonia bruguieri is a valuable source of bioactive peptides with significant antibacterial properties.
- The identified peptides show potential for development into new antimicrobial agents to combat drug-resistant bacteria.
- This study supports the use of plant resources for discovering effective natural antimicrobials.
More Related Videos
06:53Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants
Published on: May 5, 2023
10:13Production and Visualization of Bacterial Spheroplasts and Protoplasts to Characterize Antimicrobial Peptide Localization
Published on: August 11, 2018
