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An ARF1-binding factor triggering programmed cell death and periderm development in pear russet fruit skin
Yuezhi Wang1, Meisong Dai1, Xinyi Wu2
1Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Shiqiao Road No. 139, Hangzhou, Zhejiang Province, 310021, China.
Horticulture Research
|January 19, 2022
Summary
A gene deletion in PyPPCD1.1 triggers cork formation in pear fruit skin by activating phellogen. This study reveals a new molecular mechanism for periderm development and programmed cell death (PCD).
Area of Science:
- Plant biology
- Molecular genetics
- Developmental biology
Background:
- Plants possess cuticular membrane (CM) and periderm membrane (PM) for protection against environmental stresses.
- Periderm formation involves a phellogen meristem producing suberized phellem (cork), but the precise differentiation mechanism is unclear.
Purpose of the Study:
- To identify the genetic mechanism controlling phellogen differentiation and cork formation in pear fruit skin.
- To elucidate the molecular regulation of periderm development.
Main Methods:
- Map-based cloning in a pear F1 population.
- Analysis of Pyrus Periderm Programmed Cell Death 1.1 (PyPPCD1.1) gene function and protein interactions.
- Investigating gene expression and protein localization.
Main Results:
- An aspartic acid repeat deletion in PyPPCD1.1 was identified as the cause of cork formation in pear russet fruit skin.
- PyPPCD1.1 regulates programmed cell death (PCD) in an ADP-ribosylation factor 1 (ARF1)-dependent manner.
- The PyPPCD1.1 deletion affects protein localization, shifting it from the nucleus to the chloroplast.
Conclusions:
- PyPPCD1.1 acts as a switch gene controlling programmed cell death and periderm development.
- A novel molecular regulatory mechanism for periderm development has been identified in pear.
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