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Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells
Published on: February 8, 2018
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Analysis of PD-1, PD-L1, and T-cell infiltration in angiosarcoma pathogenetic subgroups
T Tomassen1, M E Weidema2, M H S Hillebrandt-Roeffen2
1Department of Pathology, Radboud University Medical Center, Nijmegen, The Netherlands.
Immunologic Research
|January 19, 2022
Summary
This study investigated programmed cell death 1 (PD-1), programmed death-ligand 1 (PD-L1), and CD8+ T cells in angiosarcoma (AS). Findings reveal differences in these markers across AS subgroups, offering prognostic insights and potential for immune checkpoint inhibition (ICI) therapy.
Area of Science:
- Oncology
- Immunology
- Genetics
Background:
- Angiosarcoma (AS) is a rare cancer with poor prognosis, often linked to radiotherapy (RT), UV radiation, or lymphoedema.
- Therapeutic progress is limited by AS heterogeneity and rarity, necessitating novel treatment strategies.
Purpose of the Study:
- To explore the potential of immune checkpoint inhibition (ICI) in AS.
- To investigate the expression of PD-1, PD-L1, and CD8+ T cells in various AS subtypes.
- To correlate these immune markers with clinical classification and methylation profiling clusters.
Main Methods:
- Protein expression analysis of PD-1, PD-L1, and CD8+ T cells in 165 AS cases.
- Correlation with clinical classifications (spontaneous, RT-associated, UV-associated, lymphoedema).
- Whole-genome methylation profiling to identify molecular clusters (A1, A2, B1, B2).
Main Results:
- High PD-L1 and PD-1 expression were common in UV-associated, visceral, and soft tissue AS.
- RT-associated AS predominantly showed high PD-1 expression.
- UV-associated AS cases in cluster A1 exhibited higher PD-1, PD-L1, and CD8+ T cells compared to cluster B2, indicating differential immunogenicity.
- Combined PD-1 and PD-L1 expression trended towards poor survival in soft tissue AS.
- PD-1 expression correlated with better survival in UV-associated AS.
Conclusions:
- The majority of AS cases express PD-1, PD-L1, and CD8+ T cells.
- Significant differences in immune marker expression exist between and within AS subgroups.
- These findings provide prognostic information and suggest predictive value for ICI therapy in specific AS subtypes.
