Dose-dependent effect of myelin oligodendrocyte glycoprotein on visual function and optic nerve damage in
Valerio Castoldi1,2, Silvia Marenna1,2, Su-Chun Huang1,2
1Experimental Neurophysiology Unit, INSPE - Institute of Experimental Neurology, San Raffaele Scientific Institute, Milan, Italy.
Abstract:
Female Dark Agouti rats were immunized with increasing doses of myelin oligodendrocyte glycoprotein (MOG) to develop experimental autoimmune encephalomyelitis (EAE), a preclinical model of multiple sclerosis. Typical EAE motor impairments were assessed daily and noninvasive visual evoked potentials (VEPs) were recorded at baseline and 5 weeks after immunization, with final histopathology of optic nerves (ONs). Immunized rats exhibited a relapsing-remitting clinical course. Both VEP and histological abnormalities were detected in a MOG dose-dependent gradient. Increasing MOG dosage augmented visual function impairment in EAE, which could be monitored with VEP recording to assess demyelination and axonal loss along ONs.
