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PCTAIRE1 promotes mitotic progression and resistance against antimitotic and apoptotic signals

Syed Qaaifah Gillani1, Irfana Reshi2, Nusrat Nabi1

  • 1Department of Biochemistry, University of Kashmir, Srinagar 190006, India.

Journal of Cell Science
|January 19, 2022
PubMed

Insights

PCTAIRE1 (CDK16) is a crucial mitotic kinase regulating cell division and centrosome function. Its role in resisting cancer cell death from antimitotic drugs highlights its potential as a therapeutic target.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • PCTAIRE1 (CDK16) is a serine-threonine kinase involved in various physiological processes.
  • Its precise function during cell division has not been fully elucidated.
  • Understanding PCTAIRE1's role is crucial for comprehending cell cycle regulation.

Purpose of the Study:

  • To investigate the role of PCTAIRE1 in cell division and mitotic progression.
  • To determine PCTAIRE1's localization and regulation during the cell cycle.
  • To explore PCTAIRE1's involvement in cancer cell resistance to antimitotic drugs.

Main Methods:

  • Library screening to identify proteins involved in mitotic arrest resistance.
  • Immunofluorescence microscopy to track PCTAIRE1 localization.
  • Cell cycle analysis and Western blotting to assess protein levels and phosphorylation.
  • PCTAIRE1 knockdown experiments to evaluate its functional impact on mitosis.
  • Assessment of cancer cell response to antimitotic drugs following PCTAIRE1 manipulation.

Main Results:

  • PCTAIRE1 was identified as a kinase that confers resistance to mitotic arrest.
  • PCTAIRE1 localizes to centrosomes, spindle poles, and the midbody during mitosis and cytokinesis.
  • PCTAIRE1 protein levels and phosphorylation peak during mitosis.
  • Knockdown of PCTAIRE1 leads to aberrant spindle assembly and chromosome segregation.
  • PCTAIRE1 enhances cancer cell resistance to antimitotic chemotherapeutic agents.

Conclusions:

  • PCTAIRE1 is a critical mediator of mitotic progression and cytokinesis.
  • PCTAIRE1 plays a significant role in conferring resistance to antimitotic drugs in cancer cells.
  • PCTAIRE1 represents a potential therapeutic target for overcoming chemotherapy resistance.
Keywords:
CDK16Chemotherapeutic resistanceMitotic arrestPCTAIRE1PLK1PP2A

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