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Updated: Oct 6, 2025
08:23
Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
Published on: February 16, 2022
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Methods to Detect Nitric Oxide and Reactive Nitrogen Species in Biological Sample
Sharanjot Kaur1, Kunj Bihari Gupta1, Sandeep Kumar1
1Department of Microbiology, School of Biological Sciences, Central University of Punjab, Bathinda, Punjab, India.
Methods in Molecular Biology (Clifton, N.J.)
|January 19, 2022
Summary
Detecting nitric oxide (NO), a key molecule in oxidative stress and diseases like cancer, is crucial. This chapter reviews common methods for NO detection and their associated challenges.
Area of Science:
- Biochemistry
- Molecular Biology
- Pathophysiology
Background:
- Oxidative stress, involving reactive oxygen species (ROS) and reactive nitrogen species (RNS), is linked to human diseases like cancer.
- Nitric oxide (NO) is a primary RNS, significantly altering cellular molecules and metabolism.
- Understanding and detecting NO generation in biological samples is vital for disease research.
Purpose of the Study:
- To summarize routinely used methods for detecting nitric oxide (NO) in biological samples.
- To highlight the challenges associated with current NO detection techniques.
Main Methods:
- Review of spectrophotometric-based assays, specifically the Griess assay.
- Review of fluorescence probe-based assays for NO detection.
- Discussion of direct and indirect NO detection methodologies.
Main Results:
- The Griess assay and fluorescence probe assays are commonly employed for NO detection.
- Various challenges exist in the accurate and reliable detection of NO using current methods.
Conclusions:
- Accurate detection of NO is essential due to its role in disease pathogenesis.
- Existing NO detection methods present challenges that require careful consideration.
- Further advancements in NO detection techniques may be necessary for improved biological and medical research.
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